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Locus name | AT5G45890 |
Alias | SAG12 | Organism | Arabidopsis thaliana | Taxonomic identifier | [NCBI] | Function category | Protein degradation/modification | Effect for Senescence | No effect | Gene Description | Senescence-associated gene 12 (SAG12) encoding a cysteine protease influenced by cytokinin, auxin, and sugars.Localized to special vacuole found during senescence called senescence associated vacuoles which are different from central vacuole in the tonoplast composition and pH. | Evidence | Molecular evidence:Northern [Ref 1]; genomic evidence: microarray data [Ref 2] | References | 1: Libault M, Wan J, Czechowski T, Udvardi M, Stacey GIdentification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.Mol. Plant Microbe Interact. 2007 Aug;20(8):900-112: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJComparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.Plant J. 2005 May;42(4):567-85 | Gene Ontology | | Sequence | AT5G45890.1 | Genomic | mRNA | CDS | Protein
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| Mutant name |
sag12-2 | Mutant/Transgenic |
mutant | Ecotype |
Col-0 |
Mutagenesis type |
T-DNA insertion_knock out |
| Sampling |
Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4) | Comparation |
Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence. |
Ortholog Group | |
| Ortholog Groups: OG5_126607 | |
Cross Link | |
| Database | Entry ID | E-value | Start | End | InterPro ID | Description | PANTHER | PTHR12411 | 1.7E-170 | 1 | 346 | IPR013128 | Peptidase C1A, papain | SUPERFAMILY | SSF54001 | 2.5E-109 | 32 | 345 | No hit | NA | SMART | SM00848 | 2.3E-21 | 38 | 96 | IPR013201 | Proteinase inhibitor I29, cathepsin propeptide | Pfam | PF08246 | 7.8E-15 | 40 | 96 | IPR013201 | Proteinase inhibitor I29, cathepsin propeptide | Pfam | PF00112 | 1.0E-81 | 130 | 345 | IPR000668 | Peptidase C1A, papain C-terminal | SMART | SM00645 | 5.6E-123 | 130 | 345 | IPR000668 | Peptidase C1A, papain C-terminal | PRINTS | PR00705 | 8.4E-10 | 148 | 163 | IPR000668 | Peptidase C1A, papain C-terminal | ProSitePatterns | PS00139 | NA | 148 | 159 | IPR000169 | Cysteine peptidase, cysteine active site | ProSitePatterns | PS00639 | NA | 287 | 297 | IPR025660 | Cysteine peptidase, histidine active site | PRINTS | PR00705 | 8.4E-10 | 289 | 299 | IPR000668 | Peptidase C1A, papain C-terminal | PRINTS | PR00705 | 8.4E-10 | 305 | 311 | IPR000668 | Peptidase C1A, papain C-terminal | ProSitePatterns | PS00640 | NA | 305 | 324 | IPR025661 | Cysteine peptidase, asparagine active site |
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| Localization 1 | Localization | vacuole | Evidence | Localization with GFP-fusion protein (pSAG12-GFP) | Pubmed ID | 15743448 |
| Localization 2 | Localization | vacuole | Evidence | SUBAcon | Pubmed ID | 23180787 |
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