Basic information
Locus name	AT4G37370
Alias	CYP81D8
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Redox regulation
Effect for Senescence	unclear
Gene Description	CYP81D8; FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; LOCATED IN: endoplasmic reticulum, plasma membrane; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, C-terminal region (InterPro:IPR017973), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: CYP81D2; electron carrier/ heme binding / iron ion binding / monooxygenase/ oxygen binding (TAIR:AT4G37360.1); Has 25196 Blast hits to 25110 proteins in 1383 species: Archae - 32; Bacteria - 3217; Metazoa - 10369; Fungi - 4683; Plants - 5696; Viruses - 3; Other Eukaryotes - 1196 (source: NCBI BLink).
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation. Plant Cell 2011 Mar;23(3):873-94
Gene Ontology	biological process oxidation-reduction process molecular function electron carrier activity heme binding iron ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Pathway	Reactome REACT_13433
Sequence	AT4G37370.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_130311	Accession Taxon NP_001117558 Arabidopsis thaliana NP_176827 Arabidopsis thaliana NP_179900 Arabidopsis thaliana NP_179903 Arabidopsis thaliana NP_181754 Arabidopsis thaliana NP_189516 Arabidopsis thaliana NP_195449 Arabidopsis thaliana NP_195450 Arabidopsis thaliana NP_195451 Arabidopsis thaliana NP_195452 Arabidopsis thaliana NP_195453 ( AT4G37370 ) Arabidopsis thaliana NP_568533 Arabidopsis thaliana NP_680150 Arabidopsis thaliana NP_001046906 Oryza sativa Japonica Group NP_001046907 Oryza sativa Japonica Group NP_001046908 Oryza sativa Japonica Group NP_001051342 Oryza sativa Japonica Group NP_001051345 Oryza sativa Japonica Group estExt_gwp_gw1.C_910162 Physcomitrella patens subsp. patens 27647.m000174 Ricinus communis 29216.m000258 Ricinus communis 29788.m000323 Ricinus communis 29910.m000948 Ricinus communis 29970.m001002 Ricinus communis 29970.m001003 Ricinus communis 30120.m000372 Ricinus communis 30170.m013773 Ricinus communis 30170.m013774 Ricinus communis 30170.m013780 Ricinus communis 30170.m014208 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR24298 2.2E-95 6 497 No hit NA Pfam PF00067 1.5E-89 31 476 IPR001128 Cytochrome P450 SUPERFAMILY SSF48264 7.7E-113 31 486 IPR001128 Cytochrome P450 PRINTS PR00463 3.1E-40 61 80 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00463 3.1E-40 85 106 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00463 3.1E-40 180 198 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00463 3.1E-40 288 305 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00385 1.9E-11 299 316 IPR001128 Cytochrome P450 PRINTS PR00463 3.1E-40 308 334 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00463 3.1E-40 351 369 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00385 1.9E-11 352 363 IPR001128 Cytochrome P450 PRINTS PR00463 3.1E-40 392 416 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00463 3.1E-40 423 433 IPR002401 Cytochrome P450, E-class, group I PRINTS PR00385 1.9E-11 424 433 IPR001128 Cytochrome P450 ProSitePatterns PS00086 NA 426 435 IPR017972 Cytochrome P450, conserved site PRINTS PR00385 1.9E-11 433 444 IPR001128 Cytochrome P450 PRINTS PR00463 3.1E-40 433 456 IPR002401 Cytochrome P450, E-class, group I
Mutant Image
SALK_129086C. This mutant exhibited normal leaf senescence.
Subcellular Localization
Localization	endoplasmic reticulum
Evidence	SUBAcon
Pubmed ID	23180787