Basic information
Locus name	AT1G73260
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Others:PCD/defense
Effect for Senescence	Unclear
Gene Description	Encodes a trypsin inhibitor involved in modulating programmed cell death in plant-pathogen interactions.
Evidence	Molecular evidence: SSH and Northern [Ref 1]; Genomic evidence:microarray data [Ref 2]
References	1: Gepstein S, Sabehi G, Carp MJ, Hajouj T, Nesher MF, Yariv I, Dor C, Bassani M Large-scale identification of leaf senescence-associated genes. Plant J. 2003 Dec;36(5):629-42 2: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	molecular function endopeptidase inhibitor activity
Sequence	AT1G73260.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G73260.1  miRNA: ath-miR172e miRNA: ath-miR172e mfe: -26.8 kcal/mol  p-value: 0.004679  position:  657  target 5' A        A         A   A 3'             AUGCAGCG UGUCGGGAU UUC              UACGUCGU GUAGUUCUA AGG   miRNA  3'          A               5'  target: AT1G73260.1  miRNA: ath-miR870 miRNA: ath-miR870 mfe: -24.2 kcal/mol  p-value: 0.014903  position:  229  target 5' C     U          U   C 3'             AUCGA GGAAACGCCA GUU              UAGCU UCUUUGUGGU UAA   miRNA  3' C                U   U 5'
Ortholog Group
Ortholog Groups: OG5_164380	Accession Taxon NP_173228 Arabidopsis thaliana NP_565061 ( AT1G73260 ) Arabidopsis thaliana NP_001053363 Oryza sativa Japonica Group 29636.m000736 Ricinus communis 29636.m000737 Ricinus communis 29636.m000738 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PRINTS PR00291 9.8E-16 28 57 IPR002160 Proteinase inhibitor I3, Kunitz legume SMART SM00452 9.1E-63 29 206 IPR002160 Proteinase inhibitor I3, Kunitz legume SUPERFAMILY SSF50386 7.7E-50 29 205 IPR011065 Kunitz inhibitor ST1-like Pfam PF00197 1.2E-38 30 204 IPR002160 Proteinase inhibitor I3, Kunitz legume ProSitePatterns PS00283 NA 30 46 IPR002160 Proteinase inhibitor I3, Kunitz legume PRINTS PR00291 9.8E-16 66 86 IPR002160 Proteinase inhibitor I3, Kunitz legume PRINTS PR00291 9.8E-16 149 168 IPR002160 Proteinase inhibitor I3, Kunitz legume PRINTS PR00291 9.8E-16 174 203 IPR002160 Proteinase inhibitor I3, Kunitz legume
Subcellular Localization
Localization 1 Localization mitochondria Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization extracellular Evidence SUBAcon Pubmed ID 23180787