Basic information
Locus name	AT1G27320
Alias	AHK3/ORE12
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Hormone response pathway:CK
Effect for Senescence	delay
Gene Description	Encodes a histidine kinases, a cytokinin receptor that controls cytokinin-mediated leaf longevity through a specific phosphorylation of the response regulator, ARR2.
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Kim HJ, Ryu H, Hong SH, Woo HR, Lim PO, Lee IC, Sheen J, Nam HG, Hwang I Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 2006 Jan 17;103(3):814-9
Gene Ontology	biological process phosphorelay signal transduction system phosphorylation regulation of transcription, DNA-dependent molecular function phosphorelay response regulator activity transferase activity, transferring phosphorus-containing groups
Pathway	Reactome REACT_14797 REACT_1046
Sequence	AT1G27320.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name ahk2-5ahk3-7 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 2 Mutant name ahk2ahk3cre1 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 3 Mutant name ahk3-3 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 4 Mutant name ahk3-7 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 5 Mutant name ahk3cre1 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 6 Mutant name ore12-1 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type EMS
miRNA Interaction
Details	target: AT1G27320.1  miRNA: ath-miR2112-3p miRNA: ath-miR2112-3p mfe: -23.6 kcal/mol  p-value: 0.054533  position:  2751  target 5' C     U   A            G 3'             UGCGC GAG GUGGG AUAAAG              ACGCG UUU CGCCU UAUUUC   miRNA  3'           A     A        5'  target: AT1G27320.1  miRNA: ath-miR773 miRNA: ath-miR773 mfe: -25.2 kcal/mol  p-value: 0.060350  position:  3114  target 5' G                 A   A 3'             GAGAU GGGGCUGGAAG AGA              CUCUG UUUCGACCUUC UUU   miRNA  3'       U           G     5'  target: AT1G27320.1  miRNA: ath-miR780 miRNA: ath-miR780 mfe: -34.0 kcal/mol  p-value: 0.003527  position:  1000  target 5' A      U          G    C 3'             ACUUGC UCAACAGCUG CUAG              UGGACG AGUUGUCGAC GAUC   miRNA  3'                        U 5'
Ortholog Group
Ortholog Groups: OG5_129304	Accession Taxon NP_564276 ( AT1G27320 ) Arabidopsis thaliana NP_565277 Arabidopsis thaliana NP_568532 Arabidopsis thaliana NP_849925 Arabidopsis thaliana NP_973396 Arabidopsis thaliana 114537 Chlamydomonas reinhardtii 97170 Chlamydomonas reinhardtii NP_001045241 Oryza sativa Japonica Group NP_001048061 Oryza sativa Japonica Group NP_001064436 Oryza sativa Japonica Group gw1.09.00.217.1 Ostreococcus tauri e_gw1.194.71.1 Physcomitrella patens subsp. patens estExt_fgenesh1_pg.C_1440014 Physcomitrella patens subsp. patens estExt_gwp_gw1.C_2710020 Physcomitrella patens subsp. patens estExt_gwp_gw1.C_500074 Physcomitrella patens subsp. patens fgenesh1_pg.scaffold_252000025 Physcomitrella patens subsp. patens fgenesh1_pg.scaffold_44000141 Physcomitrella patens subsp. patens 29597.m000294 Ricinus communis 29929.m004637 Ricinus communis 29968.m000653 Ricinus communis XP_002954901 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR24423 1.5E-138 117 1035 No hit NA ProSiteProfiles PS50839 41.1 163 389 IPR006189 CHASE SMART SM01079 4.8E-31 163 364 IPR006189 CHASE Pfam PF03924 2.4E-46 165 363 IPR006189 CHASE Coils Coil NA 427 448 No hit NA SUPERFAMILY SSF47384 2.4E-18 437 516 IPR009082 Signal transduction histidine kinase, homodimeric domain Pfam PF00512 8.4E-19 450 515 IPR003661 Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain SMART SM00388 1.3E-22 450 515 IPR003661 Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain ProSiteProfiles PS50109 42.2 457 723 IPR005467 Signal transduction histidine kinase, core SUPERFAMILY SSF55874 4.5E-40 504 596 IPR003594 Histidine kinase-like ATPase, ATP-binding domain Pfam PF02518 7.1E-30 562 720 IPR003594 Histidine kinase-like ATPase, ATP-binding domain SMART SM00387 1.5E-33 562 723 IPR003594 Histidine kinase-like ATPase, ATP-binding domain SUPERFAMILY SSF55874 4.5E-40 647 718 IPR003594 Histidine kinase-like ATPase, ATP-binding domain PRINTS PR00344 7.1E-12 648 662 IPR004358 Signal transduction histidine kinase-related protein, C-terminal PRINTS PR00344 7.1E-12 666 676 IPR004358 Signal transduction histidine kinase-related protein, C-terminal PRINTS PR00344 7.1E-12 683 701 IPR004358 Signal transduction histidine kinase-related protein, C-terminal PRINTS PR00344 7.1E-12 707 720 IPR004358 Signal transduction histidine kinase-related protein, C-terminal SUPERFAMILY SSF52172 4.4E-9 741 865 IPR011006 CheY-like superfamily SMART SM00448 0.2 745 861 IPR001789 Signal transduction response regulator, receiver domain SUPERFAMILY SSF52172 2.1E-32 889 1028 IPR011006 CheY-like superfamily SMART SM00448 4.8E-30 890 1024 IPR001789 Signal transduction response regulator, receiver domain ProSiteProfiles PS50110 29.2 891 1028 IPR001789 Signal transduction response regulator, receiver domain Pfam PF00072 2.5E-19 892 1024 IPR001789 Signal transduction response regulator, receiver domain
Mutant Image
ahk3cre1 mutants exhibited early senescence phenotype compared with wild type.
Subcellular Localization
Localization	endoplasmic reticulum
Evidence	SUBAcon
Pubmed ID	23180787