Basic information
Locus name	AT1G59750
Alias	ARF1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Hormone response pathway:Auxin
Effect for Senescence	promote
Gene Description	Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.
Evidence	Genetic evidence:Mutant [Ref 1]; Genomic evidence:microarray data [Ref 2]
References	1: Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development 2005 Oct;132(20):4563-74 2: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process regulation of transcription, DNA-dependent cellular component nucleus molecular function DNA binding
Sequence	AT1G59750.1 | Genomic | mRNA | CDS | Protein AT1G59750.2 | Genomic | mRNA | CDS | Protein AT1G59750.3 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name arf1-5 arf2-8 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 2 Mutant name arf1-4 dsARF2 Mutant/Transgenic mutant and transgene Ecotype Ws Mutagenesis type T-DNA insertion_knock out and RNAi
miRNA Interaction
Details	target: AT1G59750.1  miRNA: ath-miR400 miRNA: ath-miR400 mfe: -28.7 kcal/mol  p-value: 0.000983  position:  872  target 5' A      G              U 3'             GUGGCU GUGAUGCUUUCAUA              CACUGA UAUUAUGAGAGUAU   miRNA  3'        A                5'  target: AT1G59750.2  miRNA: ath-miR400 miRNA: ath-miR400 mfe: -28.7 kcal/mol  p-value: 0.000978  position:  872  target 5' A      G              U 3'             GUGGCU GUGAUGCUUUCAUA              CACUGA UAUUAUGAGAGUAU   miRNA  3'        A                5'  target: AT1G59750.3  miRNA: ath-miR400 miRNA: ath-miR400 mfe: -28.7 kcal/mol  p-value: 0.000910  position:  676  target 5' A      G              U 3'             GUGGCU GUGAUGCUUUCAUA              CACUGA UAUUAUGAGAGUAU   miRNA  3'        A                5'
Ortholog Group
Ortholog Groups: OG5_134025	Accession Taxon NP_001031208 ( AT1G59750 ) Arabidopsis thaliana NP_001031548 Arabidopsis thaliana NP_001031706 Arabidopsis thaliana NP_176184 Arabidopsis thaliana NP_182176 Arabidopsis thaliana NP_194129 Arabidopsis thaliana NP_201006 Arabidopsis thaliana NP_567119 Arabidopsis thaliana NP_849830 Arabidopsis thaliana NP_851244 Arabidopsis thaliana NP_973701 Arabidopsis thaliana NP_974980 Arabidopsis thaliana NP_001045270 Oryza sativa Japonica Group NP_001047137 Oryza sativa Japonica Group NP_001052879 Oryza sativa Japonica Group NP_001066765 Oryza sativa Japonica Group NP_001067988 Oryza sativa Japonica Group e_gw1.64.107.1 Physcomitrella patens subsp. patens estExt_Genewise1.C_140304 Physcomitrella patens subsp. patens estExt_Genewise1.C_2800001 Physcomitrella patens subsp. patens gw1.341.65.1 Physcomitrella patens subsp. patens 29609.m000586 Ricinus communis 29609.m000587 Ricinus communis 29742.m001397 Ricinus communis 30068.m002516 Ricinus communis 30128.m008545 Ricinus communis 30147.m014418 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR31384 2.5E-224 1 662 No hit NA SUPERFAMILY SSF101936 5.2E-44 113 253 IPR015300 DNA-binding pseudobarrel domain Pfam PF02362 1.1E-20 124 225 IPR003340 B3 DNA binding domain ProSiteProfiles PS50863 10.5 124 226 IPR003340 B3 DNA binding domain SMART SM01019 1.6E-20 124 226 IPR003340 B3 DNA binding domain Pfam PF06507 6.3E-33 250 332 IPR010525 Auxin response factor Pfam PF02309 4.0E-30 451 630 IPR003311 AUX/IAA protein ProSiteProfiles PS50962 27.7 540 622 IPR011525 Aux/IAA-ARF-dimerisation SUPERFAMILY SSF54277 2.6E-11 541 616 No hit NA
Subcellular Localization
Localization	nucleus
Evidence	SUBAcon
Pubmed ID	23180787