Basic information
Locus name	AT4G38630
Alias	RPN10/MCB1/MBP1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:26S proteasome
Effect for Senescence	delay
Gene Description	Regulatory particle non-ATPase subunit of the 26S proteasome with multiubiquitin-chain-binding capabilities.The mutant in this gene has a reduced germination rate, several growth abnormalities, increased rosette leaf senescence, a reduction in stamen number, increased sensitivity to DNA damaging agents, decreased sensitivity to cytokinin, and increased sensitivity to abscisic acid, sucrose, and salt.
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Smalle J, Kurepa J, Yang P, Emborg TJ, Babiychuk E, Kushnir S, Vierstra RD The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. Plant Cell 2003 Apr;15(4):965-80 2: van Nocker S, Deveraux Q, Rechsteiner M, Vierstra RD Arabidopsis MBP1 gene encodes a conserved ubiquitin recognition component of the 26S proteasome. Proc. Natl. Acad. Sci. U.S.A. 1996 Jan 23;93(2):856-60 3: Kurepa J, Toh-E A, Smalle JA 26S proteasome regulatory particle mutants have increased oxidative stress tolerance. Plant J. 2008 Jan;53(1):102-14
Gene Ontology	biological process ubiquitin-dependent protein catabolic process cellular component proteasome regulatory particle, base subcomplex molecular function protein binding
Sequence	AT4G38630.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	rpn10-1
Mutant/Transgenic	mutant
Ecotype	C24
Mutagenesis type	T-DNA insertion_knock out
miRNA Interaction
Details	target: AT4G38630.1  miRNA: ath-miR837-5p miRNA: ath-miR837-5p mfe: -22.1 kcal/mol  p-value: 0.077851  position:  893  target 5' G  C   U        C     A 3'             GA AAC GACAAGAA GCUGA              CU UUG UUGUUCUU UGACU   miRNA  3' A      C              A 5'
Ortholog Group
Ortholog Groups: OG5_127854	Accession Taxon NP_195575 ( AT4G38630 ) Arabidopsis thaliana CMO235C Cyanidioschyzon merolae strain 10D 81593 Chlamydomonas reinhardtii ACO68886 Micromonas sp. RCC299 NP_001049527 Oryza sativa Japonica Group gw1.02.00.425.1 Ostreococcus tauri estExt_fgenesh1_pg.C_140185 Physcomitrella patens subsp. patens 29868.m000344 Ricinus communis 29956.m000105 Ricinus communis e_gw1.14.187.1 Thalassiosira pseudonana CCMP1335 XP_002950838 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR10223 9.6E-173 1 385 IPR027040 Proteasome subunit Rpn10 SMART SM00327 1.9E-9 2 176 IPR002035 von Willebrand factor, type A SUPERFAMILY SSF53300 2.0E-36 4 174 No hit NA ProSiteProfiles PS50234 10.7 5 190 IPR002035 von Willebrand factor, type A Pfam PF13519 2.6E-11 6 185 No hit NA ProSiteProfiles PS50330 9.1 221 240 IPR003903 Ubiquitin interacting motif SMART SM00726 10.0 221 240 IPR003903 Ubiquitin interacting motif Pfam PF02809 0.0 223 236 IPR003903 Ubiquitin interacting motif Coils Coil NA 231 252 No hit NA ProSiteProfiles PS50330 8.6 282 301 IPR003903 Ubiquitin interacting motif SMART SM00726 23.0 282 301 IPR003903 Ubiquitin interacting motif Pfam PF02809 1.9E-4 305 319 IPR003903 Ubiquitin interacting motif ProSiteProfiles PS50330 10.7 305 324 IPR003903 Ubiquitin interacting motif SMART SM00726 0.0 305 324 IPR003903 Ubiquitin interacting motif
Subcellular Localization
Localization 1 Localization cytosol Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization cytosol Evidence SUBAcon Pubmed ID 23180787