Basic information
Locus name	At3g14067
Alias	SASP
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Serine protease
Effect for Senescence	No effect
Gene Description	a Senescence-Associated Subtilisin Protease
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Martinez DE, Borniego ML, Battchikova N, Aro EM, Tyystj_rvi E, Guiamut JJ. SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in Arabidopsis. J Exp Bot. 2015 Jan;66(1):161-74. doi: 10.1093/jxb/eru409. Epub 2014 Nov 4.
Gene Ontology	biological process negative regulation of catalytic activity proteolysis molecular function identical protein binding serine-type endopeptidase activity
Sequence	AT3G14067.1 | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name sasp-1, SALK_147962 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA
Mutated 2 Mutant name sasp-2, SALK_063823 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA
Ortholog Group
Ortholog Groups: OG5_129982	Accession Taxon NP_199377 Arabidopsis thaliana NP_199378 Arabidopsis thaliana NP_200789 Arabidopsis thaliana NP_563639 Arabidopsis thaliana NP_563701 Arabidopsis thaliana NP_565309 Arabidopsis thaliana NP_565330 Arabidopsis thaliana NP_566473 ( At3g14067 ) Arabidopsis thaliana NP_566483 Arabidopsis thaliana NP_567972 Arabidopsis thaliana NP_568765 Arabidopsis thaliana NP_569048 Arabidopsis thaliana NP_001043993 Oryza sativa Japonica Group NP_001046210 Oryza sativa Japonica Group NP_001047665 Oryza sativa Japonica Group NP_001048300 Oryza sativa Japonica Group NP_001048301 Oryza sativa Japonica Group NP_001048303 Oryza sativa Japonica Group NP_001048778 Oryza sativa Japonica Group NP_001049524 Oryza sativa Japonica Group NP_001050634 Oryza sativa Japonica Group NP_001051353 Oryza sativa Japonica Group NP_001053536 Oryza sativa Japonica Group NP_001053537 Oryza sativa Japonica Group NP_001053614 Oryza sativa Japonica Group NP_001061553 Oryza sativa Japonica Group NP_001061952 Oryza sativa Japonica Group NP_001063283 Oryza sativa Japonica Group NP_001063483 Oryza sativa Japonica Group NP_001064523 Oryza sativa Japonica Group NP_001065109 Oryza sativa Japonica Group NP_001066666 Oryza sativa Japonica Group e_gw1.19.32.1 Physcomitrella patens subsp. patens estExt_Genewise1.C_280065 Physcomitrella patens subsp. patens estExt_fgenesh1_pg.C_1700014 Physcomitrella patens subsp. patens fgenesh1_pm.scaffold_218000016 Physcomitrella patens subsp. patens 28348.m000079 Ricinus communis 28525.m000260 Ricinus communis 28525.m000261 Ricinus communis 29172.m000220 Ricinus communis 29637.m000731 Ricinus communis 29739.m003750 Ricinus communis 29805.m001529 Ricinus communis 29822.m003382 Ricinus communis 29836.m000560 Ricinus communis 29836.m000566 Ricinus communis 29956.m000104 Ricinus communis 30005.m001271 Ricinus communis 30026.m001511 Ricinus communis 30147.m014151 Ricinus communis 30170.m013762 Ricinus communis 30174.m008941 Ricinus communis 30190.m011072 Ricinus communis 30190.m011075 Ricinus communis 30190.m011077 Ricinus communis 30190.m011078 Ricinus communis 30190.m011082 Ricinus communis 30190.m011084 Ricinus communis 30190.m011085 Ricinus communis 30190.m011086 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR10795 0.0 1 769 IPR015500 Peptidase S8, subtilisin-related Pfam PF05922 1.2E-16 32 110 IPR010259 Proteinase inhibitor I9 SUPERFAMILY SSF52743 8.3E-83 109 378 IPR000209 Peptidase S8/S53 domain PRINTS PR00723 5.1E-18 133 152 IPR015500 Peptidase S8, subtilisin-related Pfam PF00082 2.7E-46 137 615 IPR000209 Peptidase S8/S53 domain ProSitePatterns PS00136 NA 138 149 IPR023827 Peptidase S8, subtilisin, Asp-active site PRINTS PR00723 5.1E-18 213 226 IPR015500 Peptidase S8, subtilisin-related Pfam PF02225 5.5E-13 380 464 IPR003137 Protease-associated domain, PA SUPERFAMILY SSF52025 2.5E-5 399 467 No hit NA SUPERFAMILY SSF52743 8.3E-83 490 618 IPR000209 Peptidase S8/S53 domain PRINTS PR00723 5.1E-18 543 559 IPR015500 Peptidase S8, subtilisin-related ProSitePatterns PS00138 NA 544 554 IPR023828 Peptidase S8, subtilisin, Ser-active site
Mutant Image
Mutant sasp-1 and sasp-2 plants are indistinguishable from the wild type at early stages of plant development. In spite of the senescence-associated expression of SASP, sasp-1 and sasp-2 did not show a consistent and reproducible effect on leaf senescence. However, during inflorescence development, sasp-1 and sasp-2 plants produced more branched main stems than wild-type plants.
Subcellular Localization
Localization	vacuole
Evidence	SUBAcon
Pubmed ID	23180787