Basic information
Locus name	At5g49880
Alias	NES1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Signal transduction
Effect for Senescence	delay
Gene Description	NES1 is allelic to a previously reported mitotic checkpoint family gene, MAD1
Evidence	Genetic evidence [Ref 1]
References	1: Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y. Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis. J Exp Bot. 2014 Jul;65(14):4051-63. doi: 10.1093/jxb/ert429. Epub 2013 Dec 12.
Gene Ontology	biological process mitotic spindle assembly checkpoint
Sequence	AT5G49880.1 | mRNA | CDS | Protein
Mutant information
Mutant name	nes1
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	T-DNA
Ortholog Group
Ortholog Groups: OG5_129760	Accession Taxon NP_199799 ( At5g49880 ) Arabidopsis thaliana CME139C Cyanidioschyzon merolae strain 10D 153731 Chlamydomonas reinhardtii ACO65886 Micromonas sp. RCC299 NP_001044973 Oryza sativa Japonica Group 0100010313 Ostreococcus tauri gw1.65.205.1 Physcomitrella patens subsp. patens 29609.m000583 Ricinus communis thaps1_ua_kg.chr_1000150 Thalassiosira pseudonana CCMP1335 XP_002959319 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR23168 1.3E-185 1 726 IPR008672 Spindle assembly checkpoint component Mad1 Pfam PF05557 3.0E-249 14 724 IPR008672 Spindle assembly checkpoint component Mad1 Coils Coil NA 74 102 No hit NA Coils Coil NA 148 197 No hit NA Coils Coil NA 212 247 No hit NA Coils Coil NA 278 350 No hit NA Coils Coil NA 387 408 No hit NA Coils Coil NA 429 453 No hit NA Coils Coil NA 464 534 No hit NA SUPERFAMILY SSF75704 8.5E-10 499 582 No hit NA Coils Coil NA 556 584 No hit NA Coils Coil NA 602 623 No hit NA
Mutant Image
Nitric oxide can induce premature cotyledon senescence in Arabidopsis thaliana, yet the underlying mechanism remains elusive. A screen for genetic mutants identified the nes1 mutant, in which cotyledon senescence was accelerated by nitric oxide.