Basic information
Locus name	At4g10500
Alias	SAG108/S3H
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Hormone response pathway:SA
Effect for Senescence	delay
Gene Description	Salicylic acid 3-hydroxylase (S3H) involved in SA catabolism during leaf senescence. S3H is associated with senescence and is inducible by SA and is thus a key part of a negative feedback regulation system of SA levels during senescence.
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Zhang K, Halitschke R, Yin C, Liu CJ, Gan SS. Salicylic acid 3-hydroxylase regulates Arabidopsis leaf longevity by mediating salicylic acid catabolism. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):14807-12. doi: 10.1073/pnas.1302702110. Epub 2013 Aug 19.
Gene Ontology	biological process oxidation-reduction process molecular function oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors oxidoreductase activity
Sequence	AT4G10500.1 | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name s3h Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA
Mutated 2 Mutant name s3h Mutant/Transgenic Ecotype Col-0 Mutagenesis type T-DNA
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_170611	Accession Taxon NP_192787 Arabidopsis thaliana NP_192788 ( At4g10500 ) Arabidopsis thaliana NP_001053652 Oryza sativa Japonica Group 30068.m002612 Ricinus communis 30076.m004462 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR10209 3.6E-139 11 349 No hit NA SUPERFAMILY SSF51197 2.8E-100 17 333 No hit NA Pfam PF14226 5.1E-24 44 152 IPR026992 Non-haem dioxygenase N-terminal domain ProSiteProfiles PS51471 15.3 197 296 IPR005123 Oxoglutarate/iron-dependent dioxygenase Pfam PF03171 3.2E-29 198 296 IPR005123 Oxoglutarate/iron-dependent dioxygenase
Mutant Image
The s3h knockout mutants fail to produce 2,3-DHBA sugar conjugates, accumulate very high levels of SA and its sugar conjugates, and exhibit a precocious senescence phenotype.