Basic information
Locus name	AT3G01970
Alias	WRKY45
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Transcription regulation:WRKY
Effect for Senescence	promote
Gene Description	WRKY DNA-BINDING PROTEIN 45
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Chen L, Xiang S, Chen Y, Li D, Yu D. Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf Senescence. Mol Plant. 2017 Sep 12;10(9):1174-1189. doi: 10.1016/j.molp.2017.07.008. Epub 2017 Jul 19.
Gene Ontology	biological process regulation of transcription, DNA-dependent molecular function sequence-specific DNA binding transcription factor activity sequence-specific DNA binding
Sequence	AT3G01970.1 | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name WRKY45RNAi Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type RNAi
Mutated 2 Mutant name WRKY45RNAi Mutant/Transgenic Ecotype Col-0 Mutagenesis type RNAi
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR31221 5.0E-35 49 123 No hit NA SUPERFAMILY SSF118290 2.2E-28 56 123 IPR003657 DNA-binding WRKY ProSiteProfiles PS50811 29.8 59 124 IPR003657 DNA-binding WRKY Pfam PF03106 9.1E-29 64 122 IPR003657 DNA-binding WRKY SMART SM00774 4.7E-36 64 123 IPR003657 DNA-binding WRKY
Mutant Image
Loss of WRKY45 function resulted in increased leaf longevity in age-triggered senescence, whereas overexpression of WRKY45 significantly accelerated age-triggered leaf senescence.
Subcellular Localization
Localization	nucleus
Evidence	SUBAcon
Pubmed ID	23180787