Basic information
Locus name	AT5G27420
Alias	ATL31/CNI1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	delay
Gene Description	Encodes CNI1 (Carbon/Nitrogen Insensitive1) (also named as ATL31), a RING type ubiquitin ligase that functions in the Carbon/Nitrogen response for growth phase transition in Arabidopsis seedlings.
Evidence	Genetic evidence [Ref 1]
References	1: Aoyama S, Huarancca Reyes T, Guglielminetti L, Lu Y, Morita Y, Sato T, Yamaguchi J. Ubiquitin ligase ATL31 functions in leaf senescence in response to the balance between atmospheric CO2 and nitrogen availability in Arabidopsis. Plant Cell Physiol. 2014 Feb;55(2):293-305. doi: 10.1093/pcp/pcu002. Epub 2014 Jan 6.
Gene Ontology	molecular function protein binding zinc ion binding
Sequence	AT5G27420.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name ATL31KO Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type transgenic;T-DNA
Mutated 2 Mutant name ATL31ox Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type transgenic
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_140458	Accession Taxon NP_198094 ( AT5G27420 ) Arabidopsis thaliana NP_566249 Arabidopsis thaliana CMT443C Cyanidioschyzon merolae strain 10D ACO66207 Micromonas sp. RCC299 NP_001047194 Oryza sativa Japonica Group NP_001048181 Oryza sativa Japonica Group NP_001052938 Oryza sativa Japonica Group NP_001062080 Oryza sativa Japonica Group estExt_fgenesh1_pg.C_200145 Physcomitrella patens subsp. patens fgenesh1_pg.scaffold_213000042 Physcomitrella patens subsp. patens gw1.132.48.1 Physcomitrella patens subsp. patens gw1.185.87.1 Physcomitrella patens subsp. patens gw1.257.46.1 Physcomitrella patens subsp. patens gw1.71.104.1 Physcomitrella patens subsp. patens 30128.m008738 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR22764 7.2E-51 40 170 No hit NA SUPERFAMILY SSF57850 1.3E-21 110 174 No hit NA Pfam PF13639 6.1E-17 123 167 IPR001841 Zinc finger, RING-type ProSiteProfiles PS50089 12.7 124 166 IPR001841 Zinc finger, RING-type SMART SM00184 3.0E-6 124 165 IPR001841 Zinc finger, RING-type
Mutant Image
SALK_002809C. This mutant exhibited normal leaf senescence.
Subcellular Localization
Localization	plasma membrane
Evidence	SUBAcon
Pubmed ID	23180787