Basic information
Locus name	AT4G32250
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:protein kinase and phosphatases
Effect for Senescence	unclear
Gene Description	protein kinase family protein contains protein kinase domain, Pfam:PF00069
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process protein phosphorylation molecular function ATP binding protein kinase activity
Sequence	AT4G32250.1 | Genomic | mRNA | CDS | Protein AT4G32250.2 | Genomic | mRNA | CDS | Protein AT4G32250.3 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT4G32250.2  miRNA: ath-miR854a miRNA: ath-miR854a mfe: -30.3 kcal/mol  p-value: 0.011605  position:  16  target 5' U                 U  A 3'             UUCUCUUCUCUGUCUUU UC              AGGAGGAGGGAUAGGAG AG   miRNA  3' G                 U    5'  target: AT4G32250.2  miRNA: ath-miR854b miRNA: ath-miR854b mfe: -30.3 kcal/mol  p-value: 0.013009  position:  16  target 5' U                 U  A 3'             UUCUCUUCUCUGUCUUU UC              AGGAGGAGGGAUAGGAG AG   miRNA  3' G                 U    5'  target: AT4G32250.2  miRNA: ath-miR854c miRNA: ath-miR854c mfe: -30.3 kcal/mol  p-value: 0.011519  position:  16  target 5' U                 U  A 3'             UUCUCUUCUCUGUCUUU UC              AGGAGGAGGGAUAGGAG AG   miRNA  3' G                 U    5'  target: AT4G32250.2  miRNA: ath-miR854d miRNA: ath-miR854d mfe: -30.3 kcal/mol  p-value: 0.012285  position:  16  target 5' U                 U  A 3'             UUCUCUUCUCUGUCUUU UC              AGGAGGAGGGAUAGGAG AG   miRNA  3' G                 U    5'  target: AT4G32250.2  miRNA: ath-miR854e miRNA: ath-miR854e mfe: -30.3 kcal/mol  p-value: 0.013435  position:  16  target 5' U                 U  A 3'             UUCUCUUCUCUGUCUUU UC              AGGAGGAGGGAUAGGAG AG   miRNA  3' G                 U    5'
Ortholog Group
Ortholog Groups: OG5_156308	Accession Taxon NP_001031769 Arabidopsis thaliana NP_194952 Arabidopsis thaliana NP_849560 ( AT4G32250 ) Arabidopsis thaliana 114487 Chlamydomonas reinhardtii NP_001058160 Oryza sativa Japonica Group gw1.414.6.1 Physcomitrella patens subsp. patens 29841.m002878 Ricinus communis 29848.m004612 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR22967 1.7E-246 28 370 No hit NA Pfam PF07714 3.1E-33 39 300 IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain ProSiteProfiles PS50011 30.2 39 306 IPR000719 Protein kinase, catalytic domain SUPERFAMILY SSF56112 5.1E-53 39 305 IPR011009 Protein kinase-like domain
Subcellular Localization
Localization 1 Localization cytosol Evidence SUBAcon Pubmed ID 23180787
Localization 2 Localization plastid Evidence SUBAcon Pubmed ID 23180787