CP31B - Plant Editosome Database

Summary

Editing Factor:	CP31B
Synonym:	Chloroplast RNA-binding Protein 31B
Description:	The cpRNP family member CP31A exhibit highly specific and diverse defects in chloroplast RNA metabolism; Analysis of cp31a and cp31a/cp31b double mutants uncovers that these 2 paralogous genes participate nonredundantly in a combinatorial fashion in processing a subset of chloroplast editing sites in vivo; A genome-wide analysis of chloroplast transcript accumulation in cp31a mutants detected a virtually complete loss of the chloroplast ndhF mRNA and lesser reductions for specific other mRNAs; Fluorescence analyses show that the activity of the NADH dehydrogenase complex, which also includes the NdhF subunit, is defective in cp31a mutants
Protein Family:	Chloroplast ribonucleoprotein (cpRNP)
Subclass:	NA
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	AT5G50250 (Arabidopsis thaliana)
Edited Gene(s):	psbZ rpoB ndhD
Editing Type(s):	C-to-U (10)
Publication(s):	[1] Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs, PNAS, 2009. [doi:10.1073/pnas.0808529106]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G50250

Chloroplast

ndhD

AP000423

Genomic region

116281

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	88.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	82.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

ndhD

AP000423

Genomic region

116290

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	91.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	78.00%	High	Decreased	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

psbZ

AP000423

Genomic region

35800

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	86.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	87.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

rpoB

AP000423

Genomic region

23898

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	100.00%	Complete	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	81.00%	High	Decreased	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

rpoB

AP000423

Genomic region

25779

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	79.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	84.00%	High	Similar	19297624