CP31B - Plant Editosome Database

Editing Factor:	CP31B
Synonym:	NA
Description:	Analysis of cp31a and cp31a/cp31b double mutants uncovers that these 2 paralogous genes participate nonredundantly in a combinatorial fashion in processing a subset of chloroplast editing sites in vivo; Genetic analysis of CP31A and CP31B uncovered that cpRNPs are required for specific RNA editing events and stabilize specific chloroplast mRNAs
Protein Family:	cpRNP
Subclass:	NA
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	AT5G50250 (Arabidopsis thaliana)
Edited Gene(s):	psbZ rpoB ndhD
Editing Type(s):	C-to-U (10)
Publication(s):	[1] Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs., Proceedings of the National Academy of Sciences of the United States of America, 2009. [PMID=19297624]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G50250

Chloroplast

AP000423

Genomic position

116290
116281

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	91.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	78.00%	High	Decreased	19297624
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	88.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	82.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

AP000423

Genomic position

35800

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	86.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	87.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

AP000423

Genomic position

25779
23898

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	79.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	84.00%	High	Similar	19297624
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	100.00%	Complete	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	81.00%	High	Decreased	19297624