CP31B - Plant Editosome Database

Editing Factor:	CP31B
Synonym:	NA
Description:	Analysis of cp31a and cp31a/cp31b double mutants uncovers that these 2 paralogous genes participate nonredundantly in a combinatorial fashion in processing a subset of chloroplast editing sites in vivo; Genetic analysis of CP31A and CP31B uncovered that cpRNPs are required for specific RNA editing events and stabilize specific chloroplast mRNAs
Protein Family:	cpRNP
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT5G50250 (Arabidopsis thaliana)
Edited Gene(s):	psbZ rpoB ndhD
Editing Type(s):	C-to-U (10)
Publication(s):	[1] Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs., Proceedings of the National Academy of Sciences of the United States of America, 2009. [PMID=19297624]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G50250

Chloroplast

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	91.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	78.00%	High	Decreased	19297624
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	88.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	82.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	86.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	87.00%	High	Similar	19297624

Arabidopsis thaliana

AT5G50250

Chloroplast

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	79.00%	High	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	84.00%	High	Similar	19297624
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	NA	NA	Sequencing PCR products	100.00%	Complete	None	19297624
WiscDsLox383H9	cp31b-4	T-DNA insertion	A back-to-back insertion of 2 T-DNAs at positions 1036 and 1045, respectively	Homozygous	Not affect their macroscopic appearance relative to WT under standard growth conditions	NA	NA	Sequencing PCR products	81.00%	High	Decreased	19297624