DEK36 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G21300

Mitochondrion

atp4

138

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

atp4

250

CDS

C-to-U

CCG/CCG=>UCG/UUG

P=>S/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

atp4

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

104

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

143

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

157

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

262

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

269

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

289

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

710

CDS

C-to-U

CCU/CCU=>CUU/UUU

P=>L/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

779

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

806

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn1

955

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Poor	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

176

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

208

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

226

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

259

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

277

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

320

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

344

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

356

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

391

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

ccmFn2

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

nad7

789

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

nad7

795

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

104

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

146

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

196

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

221

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

269

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

284

CDS

C-to-U

UCC/UCC=>UUC/UUU

S=>F/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Arabidopsis thaliana

AT4G21300

Mitochondrion

rps12

285

CDS

C-to-U

UCC/UCC=>UCU/UUU

S=>S/F

Synonymous/Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
WiscDsLox289_292I10.0	At_dek36	Ds insertion	Ds is inserted in 115bp	Knockout	Causes arrested embryo and endosperm development, leading to embryo lethality	Seed	12 DAP	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
Col	WT	Control	Control	Control	Normal	Seed	12 DAP	Direct Sequencing of PCR Products	NA	High	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

atp4

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Decreased	28277611
W22	W22	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	Control	Normal	Seedling	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

ccmFn

302

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
W22	W22	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	NA	High	None	28277611
Col	WT	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	NA	High	None	28277611
Col	WT	Control	Control	Control	Normal	Seedling	NA	Direct Sequencing of PCR Products	NA	High	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

nad3

230

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	28277611
Col	WT	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

nad7

383

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
W22	W22	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	Control	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	Control	Normal	Seedling	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611

Editing Factor:	DEK36
Synonym:	Defective Kernel36
Description:	Encoding an E+ subgroup PPR protein required for multiple mitochondrial transcripts editing and seed development
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	AT4G21300 (Arabidopsis thaliana) GRMZM5G892151 (Zea mays)
Edited Gene(s):	atp4 nad7 ccmFn nad3 ccmFn1 ccmFn2 rps12
Editing Type(s):	C-to-U (81)
Publication(s):	[1] E+ subgroup PPR protein defective kernel 36 is required for multiple mitochondrial transcripts editing and seed development in maize and Arabidopsis., The New phytologist, 2017. [PMID=28277611]

Summary

Editing Details