DEK36 - Plant Editosome Database

Summary

Editing Factor:	DEK36
Synonym:	Defective Kernel 36
Description:	Positional cloning reveals that DEK36, encoding a mitochondria-targeted E+ subgroup PPR protein, is required for mitochondrial RNA editing at atp4-59, nad7-383 and ccmFN -302, thus resulting in decreased activities of mitochondrial complex I, complex III and complex IV in dek36
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	GRMZM5G892151 (Zea mays)
Edited Gene(s):	atp4 nad7 ccmFn nad3
Editing Type(s):	C-to-U (17)
Publication(s):	[1] E+ subgroup PPR protein defective kernel 36 is required for multiple mitochondrial transcripts editing and seed development in maize and Arabidopsis, New Phytologist, 2017. [doi:10.1111/nph.14507]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM5G892151

Mitochondrion

atp4

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Decreased	28277611
W22	W22	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

ccmFn

302

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	NA	Medium	Decreased	28277611
W22	W22	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	NA	High	None	28277611
Col	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	NA	High	None	28277611
Col	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	NA	High	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

nad3

230

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	28277611
Col	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	28277611

Zea mays

GRMZM5G892151

Mitochondrion

nad7

383

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Kernel	NA	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
W64A * 3907l (cp-1434, o-1434)	dek36	Introgression of Mutation	G-to-E mutation in 564bp	Homozygous	Produces small and collapsed kernels, leading to embryos and/or seedlings lethality	Seedling	NA	Direct Sequencing of PCR Products	NA	Low	Decreased	28277611
W22	W22	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611
Col	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	28277611