DG1 - Plant Editosome Database

Editing Factor:	DG1
Synonym:	DELAYED GREENING 1; EMB1408; EMB246; EMBRYO DEFECTIVE 1408; EMBRYO DEFECTIVE 246; THERMO SENSITIVE LEAF COLOR 1; TSL1
Description:	Encoding a pentratricopeptide repeat containing protein that is targeted to the chloroplast; Mutants have pale young leave and reduced accumulation of plastid encoded transcripts suggesting a role for DG1 in regulation of plastid gene expression
Protein Family:	PPR
Subclass:	P
Physical Interaction:	MORF2; DYW1; DYW2
Construct Structure:	NA
Gene ID & Species:	AT5G67570 (Arabidopsis thaliana)
Edited Gene(s):	accD ndhD petL
Editing Type(s):	C-to-U (4) G-to-A (2)
Publication(s):	[1] Mutation of DELAYED GREENING1 impairs chloroplast RNA editing at elevated ambient temperature in Arabidopsis., Journal of genetics and genomics = Yi chuan xue bao, 2020. [PMID=32505546]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G67570

Chloroplast

1568

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546

Arabidopsis thaliana

AT5G67570

Chloroplast

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	65.00%	High	Decreased	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Similar	32505546

Arabidopsis thaliana

AT5G67570

Chloroplast

CDS

G-to-A

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	90.00%	High	Similar	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546