DG1 - Plant Editosome Database

Editing Factor:	DG1
Synonym:	DELAYED GREENING 1; EMB1408; EMB246; EMBRYO DEFECTIVE 1408; EMBRYO DEFECTIVE 246; THERMO SENSITIVE LEAF COLOR 1; TSL1
Description:	The missense mutation of DG1 in tsl1 mutant confers a high temperature sensitivity and impaired chloroplast development at an elevated ambient temperature in Arabidopsis; In vitro RNA electrophoretic mobility shift assay demonstrated that DG1 binds to RNA targets such as accD, ndhD, and petL
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	P
Physical Interaction:	MORF2; DYW1; DYW2
Construct Structure:	NA
Gene ID & Species:	AT5G67570 (Arabidopsis thaliana)
Edited Gene(s):	accD ndhD petL
Editing Type(s):	C-to-U (4) G-to-A (2)
Publication(s):	[1] Mutation of DELAYED GREENING1 impairs chloroplast RNA editing at elevated ambient temperature in Arabidopsis, J Genet Genomics, 2020. [doi:10.1016/j.jgg.2020.03.005]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G67570

Chloroplast

1568

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546

Arabidopsis thaliana

AT5G67570

Chloroplast

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	65.00%	High	Decreased	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Similar	32505546

Arabidopsis thaliana

AT5G67570

Chloroplast

CDS

G-to-A

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	dg1	Grown at 22℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.	Knockdown	Yellowish leaf	Leaf	NA	RT-PCR and sequencing	90.00%	High	Similar	32505546
Columbia	dg1	Grown at 29℃	A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the grow	Knockdown	Delayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.	Leaf	NA	RT-PCR and sequencing	50.00%	Medium	Decreased	32505546