Plant Editosome Database
a curated database of RNA editosome in plants

DG1 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: DG1
Synonym: DELAYED GREENING 1, DG1, EMB1408, EMB246, EMBRYO DEFECTIVE 1408, EMBRYO DEFECTIVE 246, THERMO SENSITIVE LEAF COLOR 1, TSL1
Description: Encodes a pentratricopeptide repeat containing protein that is targeted to the chloroplast. Mutants have pale young leave and reduced accumulation of plastid encoded transcripts suggesting a role for DG1 in regulation of plastid gene expression
Protein Family: PPR
Subclass: P
Construct Structure: NA
Gene ID & Species: AT5G67570 (Arabidopsis thaliana)
Edited Gene(s): accD    ndhD    petL
Editing Type(s): C-to-U (4)    G-to-A (2)
Publication(s): [1] Mutation of DELAYED GREENING1 impairs chloroplast RNA editing at elevated ambient temperature in Arabidopsis, J Genet Genomics, 2020. [PMID=32505546]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G67570 Chloroplast accD 1568 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiadg1Grown at 22℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.KnockdownYellowish leafLeafNART-PCR and sequencing50.00%MediumDecreased32505546
Columbiadg1Grown at 29℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growKnockdownDelayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.LeafNART-PCR and sequencing50.00%MediumDecreased32505546
Arabidopsis thaliana AT5G67570 Chloroplast ndhD 2 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiadg1Grown at 22℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.KnockdownYellowish leafLeafNART-PCR and sequencing65.00%HighDecreased32505546
Columbiadg1Grown at 29℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growKnockdownDelayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.LeafNART-PCR and sequencing50.00%MediumSimilar32505546
Arabidopsis thaliana AT5G67570 Chloroplast petL 5 CDS G-to-A NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiadg1Grown at 22℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then kept at 22℃ for 7 days.KnockdownYellowish leafLeafNART-PCR and sequencing90.00%HighSimilar32505546
Columbiadg1Grown at 29℃A T-DNA insertion in the 5’ untranslated region of AT5G67570, -3 bp relative to the ATG start codon, in dg1 mutant. dg1 mutant plants were germinated at 22℃ for 4 days and then transferred to the growKnockdownDelayed leaf greening under normal growth condition. Cotyledons and true leaves of dg1 appeared yellow at 29℃.LeafNART-PCR and sequencing50.00%MediumDecreased32505546
Last update: Jul 2021 (version 1.0)