DOT4 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: DOT4
Synonym: DEFECTIVELY ORGANIZED TRIBUTARIES 4; FLV
Description: Encoding a pentatricopeptide protein involved in leaf and root development. dot4 mutants have an aberrant midgap venation pattern in juvenile leaves and cotyledons
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G18750 (Arabidopsis thaliana)
Edited Gene(s): rpoC1
Editing Type(s): C-to-U (4)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Identification of two pentatricopeptide repeat genes required for RNA editing and zinc binding by C-terminal cytidine deaminase-like domains., The Journal of biological chemistry, 2013. [PMID=24194514]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G18750 Chloroplast
Plastid
rpoC1 488 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_139995dot4-2T-DNA InsertionT-DNA is inserted in repeat 5knockoutA striking phenotype of serrated white to pale green leaves, and the formation of radially symmetric, needle-shaped leavesNANABulk sequencingNAUneditedAbsent24194514
CS3254flvPoint MutationPoint mutant at Gly-554 (GGA to TGA) creates a stop codon in repeat 11NAA striking phenotype of serrated white to pale green leaves, and the formation of radially symmetric, needle-shaped leavesNANABulk sequencingNAUneditedAbsent24194514
Col-0WTControlControlControlNormalNANABulk sequencingNAMediumNone24194514
Col-0WTControlControlControlNANANARNA-seq20.79%LowNone32098170
Last update: Feb 2026 (version 2.0)