DOT4 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: DOT4
Synonym: DEFECTIVELY ORGANIZED TRIBUTARIES 4; FLV
Description: Encoding a pentatricopeptide protein involved in leaf and root development. dot4 mutants have an aberrant midgap venation pattern in juvenile leaves and cotyledons
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G18750 (Arabidopsis thaliana)
Edited Gene(s): rpoC1
Editing Type(s): C-to-U (4)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Identification of two pentatricopeptide repeat genes required for RNA editing and zinc binding by C-terminal cytidine deaminase-like domains., The Journal of biological chemistry, 2013. [PMID=24194514]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G18750 Chloroplast
Plastid
rpoC1 488 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_139995dot4-2T-DNA InsertionT-DNA is inserted in repeat 5knockoutA striking phenotype of serrated white to pale green leaves, and the formation of radially symmetric, needle-shaped leavesNANABulk sequencingNAUneditedAbsent24194514
CS3254flvPoint MutationPoint mutant at Gly-554 (GGA to TGA) creates a stop codon in repeat 11NAA striking phenotype of serrated white to pale green leaves, and the formation of radially symmetric, needle-shaped leavesNANABulk sequencingNAUneditedAbsent24194514
Col-0WTControlControlControlNormalNANABulk sequencingNAMediumNone24194514
Col-0WTControlControlControlNANANARNA-seq20.79%LowNone32098170
Last update: Jul 2021 (version 1.0)