DUA1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os09g29825

Chloroplast

rpoB

545

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Indica	dua1	At low temperature; Mutation	At 19℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	10.00%	Poor	Decreased	30295937
Nipponbare	nip	At low temperature	At 19℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937
Indica	cp1	At low temperature; Complementation	At 19℃; A complementation experiment was conducted by transforming the dua1 calli. We amplified the DUA1 full‐length ORF using a cDNA clone (J043016D20) as a template. Under the activity of the actin	Recessive; Heterozygous	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	90.00%	High	Restored	30295937
Indica	dua1	At normal temperature; Mutation	At 30℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	50.00%	Medium	Decreased	30295937
Nipponbare	nip	At normal temperature	At 30℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937

Oryza sativa

LOC_Os09g29825

Chloroplast

rpoB

560

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Indica	dua1	At low temperature; Mutation	At 19℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	0.00%	Unedited	Absent	30295937
Nipponbare	nip	At low temperature	At 19℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937
Indica	cp1	At low temperature; Complementation	At 19℃; A complementation experiment was conducted by transforming the dua1 calli. We amplified the DUA1 full‐length ORF using a cDNA clone (J043016D20) as a template. Under the activity of the actin	Recessive; Heterozygous	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	85.00%	High	Restored	30295937
Indica	dua1	At normal temperature; Mutation	At 30℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	50.00%	Medium	Decreased	30295937
Nipponbare	nip	At normal temperature	At 30℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937

Oryza sativa

LOC_Os09g29825

Chloroplast

rps8

182

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Indica	dua1	At low temperature; Mutation	At 19℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	0.00%	Unedited	Absent	30295937
Nipponbare	nip	At low temperature	At 19℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937
Indica	cp1	At low temperature; Complementation	At 19℃; A complementation experiment was conducted by transforming the dua1 calli. We amplified the DUA1 full‐length ORF using a cDNA clone (J043016D20) as a template. Under the activity of the actin	Recessive; Heterozygous	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	90.00%	High	Restored	30295937
Indica	dua1	At normal temperature; Mutation	At 30℃; An eight‐nucleotide deletion 58 bp downstream of ATG resulted in a frameshift mutation in dua1. A base change from ‘T’ to ‘C’ upstream of the ORF.	Recessive; Heterozygous	A pale leaf phenotype	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	Similar	30295937
Nipponbare	nip	At normal temperature	At 30℃	No mutant	Normal pigment content	Leaf	Third‐leaf‐stage	PCR sequencing	100.00%	Complete	None	30295937

Editing Factor:	DUA1
Synonym:	NA
Description:	Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures; DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low-temperature conditions; Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-DYW
Gene ID & Species:	LOC_Os09g29825 (Oryza sativa)
Edited Gene(s):	rpoB rps8
Editing Type(s):	C-to-U (15)
Publication(s):	[1] The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice., The New phytologist, 2019. [PMID=30295937]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details