Dek570‑1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

301

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	0.00%	Unedited	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	70.00%	High	Increased	39985592

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

306

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	77.00%	High	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	16.00%	Poor	Decreased	39985592

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

799

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	39985592

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	50.00%	Medium	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	39985592

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

906

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	50.00%	Medium	Decreased	39985592

Zea mays

Zm00001d028422

Mitochondrion

ccmFc

Transcript

966

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	Similar	39985592

Zea mays

Zm00001d028422

Mitochondrion

nad2

Transcript

677

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	39985592

Zea mays

Zm00001d028422

Chloroplast

rpl20

CDS

Transcript

308

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zheng58	Zheng58	Control	Control	No mutant	Normal	Kernel	NA	RT-PCR products were directly sequenced	100.00%	Complete	None	39985592
Zheng58	dek570-1	A G-to-A substitution	Sequencing analysis revealed that a G-to-A substitution at 966 bp downstream of the starting codon ATG of Zm00001d028422 in the dek570-1 mutant	Heterozygous; Recessive	dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm; dek570-1 plants are short and yellowed, but they can reproduce offspring	Kernel	NA	RT-PCR products were directly sequenced	64.00%	High	Decreased	39985592

Editing Factor:	Dek570‑1
Synonym:	Defective Kernel 570-1; PPR22; Pentatricopeptide Repeat Protein 22
Description:	The expression of some rRNAs, plastid-encoded RNA polymerase (PEP)- and nuclearencoded single-subunit RNA polymerase (NEP)-dependent genes, thereby reducing chlorophyll accumulation and photosynthetic rate; Excessive ROS accumulation in the dek570‑1 mutant
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	Zm00001d028422 (Zea mays)
Edited Gene(s):	ccmFc nad2 rpl20
Editing Type(s):	C-to-U (16)
Publication(s):	[1] Dek570-1, a PPR-DYW protein, is required for maize seed and plant development via modulation of C-to-U RNA editing in mitochondria and chloroplasts, Planta, 2025. [doi:10.1007/s00425-025-04634-z]

Summary

Editing Details