EMB2261 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: EMB2261
Synonym: ECD1, EMB2261, EMBRYO DEFECTIVE 2261
Description: Tetratricopeptide repeat (TPR)-like superfamily protein;(source:Araport11)
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: AT3G49170 (Arabidopsis thaliana)
Edited Gene(s): rps14-2    rps14
Editing Type(s): C-to-U (4)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development, Front Plant Sci, 2018. [PMID=29973946]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G49170 Plastid rps14 194 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq92.63%HighNone32098170
Arabidopsis thaliana AT3G49170 Chloroplast rps14-2 37092 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentGreen seedsSeedling18 daysRNA-seq82.00%HighNone29973946
SALK_024975 ABI3:EMB2261A T-DNA insertion and complementation A T-DNA insertion was mapped directly after the nucleotide 1809 of the EMB2261 gene, within the region encoding the L2 motif. The EMB2261 gene fragment was cloned from the entry vector pDONR207 to theHomozygousShorter than WT or as high as WTSeedling18 daysRNA-seq49.00%MediumDecreased29973946
SALK_024975 35S:EMB2261A T-DNA insertion and complementation A T-DNA insertion was mapped directly after the nucleotide 1809 of the EMB2261 gene, within the region encoding the L2 motif. The EMB2261 gene fragment was cloned from the entry vector pDONR207 to theHomozygousShorter than WT or as high as WTSeedling18 daysRNA-seq97.00%HighRestored29973946
Last update: Jul 2021 (version 1.0)