EMB2261 - Plant Editosome Database

Editing Factor:	EMB2261
Synonym:	ECD1; EMBRYO DEFECTIVE 2261
Description:	The emb2261-2 mutation is lethal through a failure to express an editing factor that specifically recognizes the rps14-2 site; EMB2261 was predicted to bind the cis-element upstream of the rps14-2 site and genetic complementation with promoters of different strength followed by RNA-seq analysis was conducted to test the correlation between rps14-2 editing and EMB2261 expression; Rps14-2 is the only editing event in Arabidopsis chloroplasts that correlates with EMB2261 expression; Sequence divergence between the cis-element and the EMB2261 protein sequence in plants where rps14-2 editing is not required adds support to the association between them
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	AT3G49170 (Arabidopsis thaliana)
Edited Gene(s):	rps14
Editing Type(s):	C-to-U (3)
Publication(s):	[1] Editing of Chloroplast rps14 by PPR Editing Factor EMB2261 Is Essential for Arabidopsis Development, Front Plant Sci, 2018. [doi:10.3389/fpls.2018.00841]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G49170

Chloroplast

37092

CDS

TAIR (v10)

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Green seeds	Seedling	18 days	RNA-seq	82.00%	High	None	29973946
SALK_024975	ABI3:EMB2261	A T-DNA insertion and complementation	A T-DNA insertion was mapped directly after the nucleotide 1809 of the EMB2261 gene, within the region encoding the L2 motif. The EMB2261 gene fragment was cloned from the entry vector pDONR207 to the	Homozygous	Shorter than WT or as high as WT	Seedling	18 days	RNA-seq	49.00%	Medium	Decreased	29973946
SALK_024975	35S:EMB2261	A T-DNA insertion and complementation	A T-DNA insertion was mapped directly after the nucleotide 1809 of the EMB2261 gene, within the region encoding the L2 motif. The EMB2261 gene fragment was cloned from the entry vector pDONR207 to the	Homozygous	Shorter than WT or as high as WT	Seedling	18 days	RNA-seq	97.00%	High	Restored	29973946