| Editing Factor: | EMB2261 |
| Synonym: | ECD1; EMBRYO DEFECTIVE 2261 |
| Description: | The emb2261-2 mutation is lethal through a failure to express an editing factor that specifically recognizes the rps14-2 site; EMB2261 was predicted to bind the cis-element upstream of the rps14-2 site and genetic complementation with promoters of different strength followed by RNA-seq analysis was conducted to test the correlation between rps14-2 editing and EMB2261 expression; Rps14-2 is the only editing event in Arabidopsis chloroplasts that correlates with EMB2261 expression; Sequence divergence between the cis-element and the EMB2261 protein sequence in plants where rps14-2 editing is not required adds support to the association between them |
| Protein Family: | PPR |
| Subclass: | DYW |
| Construct Structure: | PLS-E1-E2-DYW |
| Gene ID & Species: | AT3G49170 (Arabidopsis thaliana) |
| Edited Gene(s): | rps14 |
| Editing Type(s): | C-to-U (4) |
| Publication(s): |
[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Editing of Chloroplast by PPR Editing Factor EMB2261 Is Essential for Development., Frontiers in plant science, 2018. [PMID=29973946] |
| Species | Gene ID | Organelle | Edited Gene | Position | Region | Editing Type | Codon | Amino Acid | Molecular Effect | Experiment Details | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Arabidopsis thaliana | AT3G49170 | Plastid | rps14 | 194 | CDS | C-to-U | NA=>NA | NA=>NA | NA |
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| Arabidopsis thaliana | AT3G49170 | Chloroplast | rps14 | 37092 | CDS | C-to-U | CCA=>CUA | P=>L | Recoding |
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