PED
Plant
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EMP5 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: EMP5
Synonym: Empty Pericarp 5
Description: Analysis of the mitochondrial transcripts revealed that loss of the EMP5 function abolishes the C-to-U editing of ribosomal protein L16 at the rpl16-458 site (100% edited in the wild type), decreases the editing at nine sites in NADH dehydrogenase9 (nad9), cytochrome c oxidase3 (cox3), and ribosomal protein S12 (rps12), and surprisingly increases the editing at five sites of ATP synthase F0 subunit a (atp6), apocytochrome b (cob), nad1, and rpl16; Mutant EMP5-4 lacking the E+ and DYW domains still retains the substrate specificity and editing function, only at reduced efficiency
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: LOC_Os01g72930 (Oryza sativa)
Edited Gene(s): rpl16
Editing Type(s): C-to-U (4)
Publication(s): [1] Empty pericarp5 encodes a pentatricopeptide repeat protein that is required for mitochondrial RNA editing and seed development in maize, The Plant Cell, 2013. [doi:10.1105/tpc.112.106781]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_Os01g72930 Mitochondrion rpl16 458 CDS NA NA NA NA C-to-U CUA=>CCA L=>P Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NipponbareOsEmp5-RNAi transgenic lines 19RNAiRNAi vector, pTCK303-OsEmp5, is introduced into the wild-type cultivar Nipponbare using Agrobacterium-mediated transformationNAThe transgenic plants grew much slower than the wild type at seedling stage. But after the seedling stage, the plants gradually recovered and grew to normal adult plantsLeafNADirect Sequencing of PCR ProductsNALowDecreased23463776
NipponbareOsEmp5-RNAi transgenic lines 23RNAiRNAi vector, pTCK303-OsEmp5, is introduced into the wild-type cultivar Nipponbare using Agrobacterium-mediated transformationNAThe transgenic plants grew much slower than the wild type at seedling stage. But after the seedling stage, the plants gradually recovered and grew to normal adult plantsLeafNADirect Sequencing of PCR ProductsNAMediumDecreased23463776
NipponbareOsEmp5-RNAi transgenic lines 33RNAiRNAi vector, pTCK303-OsEmp5, is introduced into the wild-type cultivar Nipponbare using Agrobacterium-mediated transformationNAThe transgenic plants grew much slower than the wild type at seedling stage. But after the seedling stage, the plants gradually recovered and grew to normal adult plantsLeafNADirect Sequencing of PCR ProductsNAMediumDecreased23463776
NipponbareWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone23463776
Last update: Sep 2026 (version 2.0)