LOI1 - Plant Editosome Database

Summary

Editing Factor:	LOI1
Synonym:	LOVASTATIN INSENSITIVE 1; MEF11; MITOCHONDRIAL RNA EDITING FACTOR 11
Description:	Encoding a pentatricopeptide (PPR) protein that binds single-stranded RNA; The N-terminal portion of the protein can localize to the mitochondria; Mutations in this gene make plants less sensitive to inhibitors of the MEP and MVA pathways of isoprenoid biosynthesis and increase the activity of HMG CoA reductase
Protein Family:	PPR
Subclass:	DYW,NA
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW, NA
Gene ID & Species:	AT4G14850 (Arabidopsis thaliana)
Edited Gene(s):	nad4 ccmFn2 cox3 NA
Editing Type(s):	C-to-U (9) NA (4)
Publication(s):	[1] The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing., The Plant journal : for cell and molecular biology, 2010. [PMID=19929879] [2] Lovastatin insensitive 1, a Novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis., Plant & cell physiology, 2007. [PMID=17213228]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G14850

Mitochondrion

ccmFn2

344

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Loi1Promotor::Loi1/loi1-1	Complementation	loi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 gene	NA	NA	Seedling	2 weeks	Direct Sequencing of PCR Products	NA	High	Restored	19929879
Col-0	loi1-1	T-DNA Insertion	T-DNA is inserted at site 542bp	NA	Lower sensitivity to lovastatin and clomazone	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19929879
Col-0	WT	Control	Control	Control	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19929879

Arabidopsis thaliana

AT4G14850

Mitochondrion

cox3

422

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Loi1Promotor::Loi1/loi1-1	Complementation	loi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 gene	NA	NA	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19929879
Col-0	loi1-1	T-DNA Insertion	T-DNA is inserted at site 542bp	NA	Lower sensitivity to lovastatin and clomazone	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19929879
Col-0	WT	Control	Control	Control	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19929879

Arabidopsis thaliana

AT4G14850

Mitochondrion

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	Root elongation in WT plants grown with 300 nM lovastatin was strongly reduced	Root	NA	NA	NA	NA	NA	17213228
Col-0	loi1-1	T-DNA insertion	A T-DNA insertion was at +542bp in the first exon of the At4g14850 gene	Homozygous	The root elongation of treated loi1-1 plants was less affected	Root	NA	NA	NA	NA	NA	17213228
Col-0	loi1-2	T-DNA insertion	A T-DNA insertion was at +729bp in the first exon of the At4g14850 gene	NA	The root elongation of treated loi1-2 plants was less affected	Root	NA	NA	NA	NA	NA	17213228
Col-0	35S:: LOI1 in loi1-1	T-DNA insertion	A T-DNA insertion was at +542bp in the first exon of the At4g14850 gene. A DNA fragment containing the first 39 amino acid residues of the LOI1 protein and five residues of the glycine linker was amp	Homozygous	Root elongation in loi1-1(35S:: LOI1) plants grown with 300 nM lovastatin was strongly reduced	Root	NA	NA	NA	NA	NA	17213228

Arabidopsis thaliana

AT4G14850

Mitochondrion

nad4

124

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Loi1Promotor::Loi1/loi1-1	Complementation	loi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 gene	NA	NA	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19929879
Col-0	loi1-1	T-DNA Insertion	T-DNA is inserted at site 542bp	NA	Lower sensitivity to lovastatin and clomazone	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19929879
Col-0	WT	Control	Control	Control	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19929879