Plant Editosome Database
a curated database of RNA editosome in plants

LOI1 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: LOI1
Synonym: LOVASTATIN INSENSITIVE 1; MEF11; MITOCHONDRIAL RNA EDITING FACTOR 11
Description: Encoding a pentatricopeptide (PPR) protein that binds single-stranded RNA; The N-terminal portion of the protein can localize to the mitochondria; Mutations in this gene make plants less sensitive to inhibitors of the MEP and MVA pathways of isoprenoid biosynthesis and increase the activity of HMG CoA reductase
Protein Family: PPR
Subclass: DYW,NA
Construct Structure: PLS-E-DYW, NA
Gene ID & Species: AT4G14850 (Arabidopsis thaliana)
Edited Gene(s): nad4    ccmFn2    cox3    NA
Editing Type(s): C-to-U (9)    NA (4)
Publication(s): [1] The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing., The Plant journal : for cell and molecular biology, 2010. [PMID=19929879]
[2] Lovastatin insensitive 1, a Novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis., Plant & cell physiology, 2007. [PMID=17213228]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G14850 Mitochondrion ccmFn2 344 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Loi1Promotor::Loi1/loi1-1Complementationloi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 geneNANASeedling2 weeksDirect Sequencing of PCR ProductsNAHighRestored19929879
Col-0loi1-1T-DNA InsertionT-DNA is inserted at site 542bpNALower sensitivity to lovastatin and clomazoneSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent19929879
Col-0WTControlControlControlNormalSeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteNone19929879
Arabidopsis thaliana AT4G14850 Mitochondrion cox3 422 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Loi1Promotor::Loi1/loi1-1Complementationloi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 geneNANASeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteRestored19929879
Col-0loi1-1T-DNA InsertionT-DNA is inserted at site 542bpNALower sensitivity to lovastatin and clomazoneSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent19929879
Col-0WTControlControlControlNormalSeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteNone19929879
Arabidopsis thaliana AT4G14850 Mitochondrion NA NA NA NA NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlRoot elongation in WT plants grown with 300 nM lovastatin was strongly reducedRootNANANANANA17213228
Col-0loi1-1T-DNA insertionA T-DNA insertion was at +542bp in the first exon of the At4g14850 geneHomozygousThe root elongation of treated loi1-1 plants was less affectedRootNANANANANA17213228
Col-0loi1-2T-DNA insertionA T-DNA insertion was at +729bp in the first exon of the At4g14850 geneNAThe root elongation of treated loi1-2 plants was less affectedRootNANANANANA17213228
Col-035S:: LOI1 in loi1-1T-DNA insertionA T-DNA insertion was at +542bp in the first exon of the At4g14850 gene. A DNA fragment containing the first 39 amino acid residues of the LOI1 protein and five residues of the glycine linker was ampHomozygousRoot elongation in loi1-1(35S:: LOI1) plants grown with 300 nM lovastatin was strongly reducedRootNANANANANA17213228
Arabidopsis thaliana AT4G14850 Mitochondrion nad4 124 CDS C-to-U CUG=>UUG L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Loi1Promotor::Loi1/loi1-1Complementationloi1-1 is complemented by the introduction of the gene cassette of the LOI1 promoter::LOI1 geneNANASeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteRestored19929879
Col-0loi1-1T-DNA InsertionT-DNA is inserted at site 542bpNALower sensitivity to lovastatin and clomazoneSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent19929879
Col-0WTControlControlControlNormalSeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteNone19929879
Last update: Jul 2021 (version 1.0)