Plant Editosome Database
a curated database of RNA editosome in plants

MEF26 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF26
Synonym: Mitochondrial Editing Factor 26
Description: Encodes a DYW-PPR protein involved in mitochondrial RNA editing at two sites.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G03580 (Arabidopsis thaliana)
Edited Gene(s): cox3    nad4
Editing Type(s): C-to-U (18)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The pentatricopeptide repeat protein MEF26 participates in RNA editing in mitochondrial cox3 and nad4 transcripts, Mitochondrion, 2014. [PMID=25173472]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G03580 Mitochondrion cox3 311 CDS C-to-U UCU=>UUU
NA=>NA		 S=>F
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteNone25173472
LerLerNo treatmentNo treatmentNo treatmentNo treatmentLeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteNone25173472
GT_5_22445mef26-1Ds insertionDs is located at 18 bpHomozygousNALeaf6 weeksDirect Sequencing of PCR Products20.00%LowDecreased25173472
GK_895H11mef26-2T-DNA InsertionT-DNA is inserted at 947 bp downstream of the ATG codonHomozygousNALeaf6 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent25173472
GK_895H11mef26-2+MEF26ComplementationMEF26 coding region is first cloned into pENTR/D-TOPO (Invitrogen) and then recloned into the binary vector pMDC32, between the CaMV35S promoter and the NOS terminatorNANALeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteRestored25173472
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq96.83%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT3G03580 Mitochondrion nad4 166 CDS C-to-U CGG=>UGG
NA=>NA		 R=>W
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteNone25173472
LerLerNo treatmentNo treatmentNo treatmentNo treatmentLeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteNone25173472
GT_5_22445mef26-1Ds insertionDs is located at 18 bpHomozygousNALeaf6 weeksDirect Sequencing of PCR Products55.00%MediumDecreased25173472
GK_895H11mef26-2T-DNA InsertionT-DNA is inserted at 947 bp downstream of the ATG codonHomozygousNALeaf6 weeksDirect Sequencing of PCR Products60.00%MediumDecreased25173472
GK_895H11mef26-2+MEF26ComplementationMEF26 coding region is first cloned into pENTR/D-TOPO (Invitrogen) and then recloned into the binary vector pMDC32, between the CaMV35S promoter and the NOS terminatorNANALeaf6 weeksDirect Sequencing of PCR Products100.00%CompleteRestored25173472
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.55%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)