MEF26 - Plant Editosome Database

Summary

Editing Factor:	MEF26
Synonym:	MITOCHONDRIAL EDITING FACTOR 26
Description:	While at one site, cox3-311, editing is abolished in the absence of MEF26, the other site, nad4-166, is still partially edited; These sites share similar cis-elements and application of the recently proposed amino acid code for RNA recognition by PPR proteins ranks them at first and second positions of the most probable targets; MEF26 orthologs are not present in all plant species able to edit these sites
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT3G03580 (Arabidopsis thaliana)
Edited Gene(s):	cox3 nad4
Editing Type(s):	C-to-U (16)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The pentatricopeptide repeat protein MEF26 participates in RNA editing in mitochondrial cox3 and nad4 transcripts, Mitochondrion, 2014. [doi:10.1016/j.mito.2014.08.006]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G03580

Mitochondrion

cox3

311

CDS

TAIR (http://www.arabidopsis.org)
NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	25173472
Ler	Ler	Control	Control	No mutant	Normal	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	25173472
GT_5_22445	mef26-1	Ds insertion	Ds is located at 18 bp	Homozygous	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	20.00%	Low	Decreased	25173472
GK_895H11	mef26-2	T-DNA Insertion	T-DNA is inserted at 947 bp downstream of the ATG codon	Homozygous	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25173472
GK_895H11	mef26-2+MEF26	Complementation	MEF26 coding region is first cloned into pENTR/D-TOPO (Invitrogen) and then recloned into the binary vector pMDC32, between the CaMV35S promoter and the NOS terminator	NA	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	25173472
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT3G03580

Mitochondrion

nad4

166

CDS

TAIR (http://www.arabidopsis.org)
NA

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	25173472
Ler	Ler	Control	Control	No mutant	Normal	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	25173472
GT_5_22445	mef26-1	Ds insertion	Ds is located at 18 bp	Homozygous	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	55.00%	Medium	Decreased	25173472
GK_895H11	mef26-2	T-DNA Insertion	T-DNA is inserted at 947 bp downstream of the ATG codon	Homozygous	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	60.00%	High	Decreased	25173472
GK_895H11	mef26-2+MEF26	Complementation	MEF26 coding region is first cloned into pENTR/D-TOPO (Invitrogen) and then recloned into the binary vector pMDC32, between the CaMV35S promoter and the NOS terminator	NA	NA	Leaf	6 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	25173472
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003