MEF3 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF3
Synonym: MITOCHONDRIAL EDITING FACTOR 3
Description: Encoding a sequence-specific RNA editing factor involved in editing of the atp4-89 site in plant mitochondria.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT1G06140 (Arabidopsis thaliana)
Edited Gene(s): atp4
Editing Type(s): C-to-U (9)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The E-class PPR protein MEF3 of Arabidopsis thaliana can also function in mitochondrial RNA editing with an additional DYW domain., Plant & cell physiology, 2012. [PMID=22186180]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G06140 Mitochondrion atp4 89 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
LerWTControlControlControlNormalLeafNADirect Sequencing of PCR Products50.00%MediumNone22186180
C24WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
NossenWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
Nossenmef3-1T-DNA InsertionT-DNA is inserted in repeat 10NANALeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22186180
Col-0WTControlControlControlNANANARNA-seq99.21%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)