MEF3 - Plant Editosome Database

Summary

Editing Factor:	MEF3
Synonym:	MITOCHONDRIAL EDITING FACTOR 3
Description:	In Vitis vinifera the gene most similar to MEF3 continues into a DYW extension beyond the common E domain; The additional DYW domain does not disturb the MEF3 protein function in mitochondrial RNA editing in A. thaliana
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT1G06140 (Arabidopsis thaliana)
Edited Gene(s):	atp4
Editing Type(s):	C-to-U (8)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The E-Class PPR Protein MEF3 of Arabidopsis thaliana Can Also Function in Mitochondrial RNA Editing With an Additional DYW Domain, Plant Cell Physiology, 2012. [doi:10.1093/pcp/pcr182]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G06140

Mitochondrion

atp4

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22186180
Ler	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	22186180
C24	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22186180
Nossen	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22186180
Nossen	mef3-1	T-DNA Insertion	T-DNA is inserted in repeat 10	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22186180
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	94.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	93.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	92.00%	High	Similar	28761003