MEF35 - Plant Editosome Database

Summary

Editing Factor:	MEF35
Synonym:	MITOCHONDRIAL EDITING FACTOR 35
Description:	The MEF35 protein contains eleven PPR repeats and E and DYW extensions at the C-terminus; Two T-DNA insertion mutants, one inserted just upstream and the other inside the reading frame encoding the DYW domain, show loss of editing at a site in each of the mRNAs for protein 16 in the large ribosomal subunit (site rpl16-209), for cytochrome b (cob-286) and for subunit 4 of complex I (nad4-1373), respectively; Editing is restored upon introduction of the wild type MEF35 gene in the reading frame mutant; Homozygous mutant plants develop indistinguishably from wild type plants, although the RPL16 and COB/CYTB proteins are essential and the amino acids encoded after the editing events are conserved in most plant species
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	MORF1; MORF8
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT4G14050 (Arabidopsis thaliana)
Edited Gene(s):	rpl16 nad4 cob
Editing Type(s):	C-to-U (21)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The DYW Subgroup PPR Protein MEF35 Targets RNA Editing Sites in the Mitochondrial rpl16, nad4 and cob mRNAs in Arabidopsis thaliana, PLOS One, 2015. [doi:10.1371/journal.pone.0140680]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G14050

Mitochondrion

cob

286

CDS

C-to-U

CUU=>UUU
NA=>NA

L=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	26470017
SAIL_101_B01	mef35-1	T-DNA Insertion	T-DNA is inserted in the upstream	Homozygous	The plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WT	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2	T-DNA Insertion	T-DNA is inserted in the reading frame encoding the DYW domain	Homozygous	Seedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stage	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2+MEF35	Complementation	The MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123	Homozygous	Retarded growth	NA	NA	Direct Sequencing of PCR Products	NA	High	Restored	26470017
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT4G14050

Mitochondrion

nad4

1373

CDS

C-to-U

UCC=>UUC
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	26470017
SAIL_101_B01	mef35-1	T-DNA Insertion	T-DNA is inserted in the upstream	Homozygous	The plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WT	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2	T-DNA Insertion	T-DNA is inserted in the reading frame encoding the DYW domain	Homozygous	Seedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stage	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2+MEF35	Complementation	The MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123	Homozygous	Retarded growth	NA	NA	Direct Sequencing of PCR Products	NA	High	Restored	26470017
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	Similar	28761003

Arabidopsis thaliana

AT4G14050

Mitochondrion

rpl16

209

CDS

C-to-U

ACU=>AUU
NA=>NA

T=>I
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	NA	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	26470017
SAIL_101_B01	mef35-1	T-DNA Insertion	T-DNA is inserted in the upstream	Homozygous	The plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WT	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2	T-DNA Insertion	T-DNA is inserted in the reading frame encoding the DYW domain	Homozygous	Seedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stage	NA	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	26470017
SALK_060464	mef35-2+MEF35	Complementation	The MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123	Homozygous	Retarded growth	NA	NA	Direct Sequencing of PCR Products	NA	High	Restored	26470017
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	96.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003