MORF8 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

128

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100.00%	Complete	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	59.37%	Medium	Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

148, 149

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CCG=>UUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100%, 100%	Complete, Complete	None, None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	15.07%, 19.32%	Poor, Poor	Decreased, Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

164

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100.00%	Complete	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	36.23%	Low	Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

172

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	92.50%	High	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	18.35%	Poor	Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

193, 194

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CCU=>UUU

P=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100%, 100%	Complete, Complete	None, None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	74.07%, 89.50%	High, High	Decreased, Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

ccb206

286

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100.00%	Complete	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	67.80%	High	Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Mitochondrion

cob

853

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100.00%	Complete	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	62.17%	High	Decreased	32972981

Nicotiana benthamiana

Niben101Scf20512g00013.1

Chloroplast

ndhB

242

CDS

Sol Genomics Network (https://solgenomics.net)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	TRV-GFP	Control	Control	No mutant	Normal	Leaf	NA	Sequencing PCR products	100.00%	Complete	None	32972981
NA	TRV-NbMORF8	NbMORF8 silencing	NA	NA	Silencing NbMORF8 conferred enhanced resistance to all tested Phytophthora pathogens	Leaf	NA	Sequencing PCR products	86.14%	High	Decreased	32972981

Editing Factor:	MORF8
Synonym:	MULTIPLE ORGANELLAR RNA EDITING FACTOR 8; RIP1; RNA-EDITING FACTOR INTERACTING PROTEIN 1
Description:	Editing assays for all 34 known Arabidopsis chloroplast targets in a rip1 mutant revealed altered efficiency of 14 editing events; In mitochondria, 266 editing events were found to have reduced efficiency, with major loss of editing at 108 C targets; he presence of RIP1 in a protein complex along with chloroplast editing factor RARE1 indicates that RIP1 is an important component of the RNA editing apparatus that acts on many chloroplast and mitochondrial C targets
Protein Family:	Multiple organellar RNA editing factor (MORF)
Subclass:	NA
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	Niben101Scf20512g00013.1 (Nicotiana benthamiana)
Edited Gene(s):	cob ndhB ccb206
Editing Type(s):	C-to-U (16)
Publication(s):	[1] Cytidine-to-Uridine RNA Editing Factor NbMORF8 Negatively Regulates Plant Immunity to Phytophthora Pathogens, Plant Physiol, 2020. [doi:10.1104/pp.20.00458]

Summary

Editing Details