MORF9.3 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Camellia sinensis

CSS0008579

Chloroplast

matK

445

CDS

TPIA (https://tpia.teaplants.cn)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	pTRV2	Infected with pTRV1+pTRV2 Agrobacterium	NA	No mutant	Normal	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	78.00%	High	None	42159751
NA	pTRV2-CsMORF9.3	VIGS treatment	The pTRV2-CsMORF9.3-silencing plasmid was constructed by inserting a 300 bp cDNA fragment of CsMORF9.3 into the pTRV2 viral vector	No mutant	The newly developed shoots and leaves exhibited a yellowing or pale-green phenotype; The chlorophyll fluorescence parameter Fv/Fm also showed a significant reduction	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	100.00%	Complete	Increased	42159751

Camellia sinensis

CSS0008579

Chloroplast

ndhD

1310

CDS

TPIA (https://tpia.teaplants.cn)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	pTRV2	Infected with pTRV1+pTRV2 Agrobacterium	NA	No mutant	Normal	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	100.00%	Complete	None	42159751
NA	pTRV2-CsMORF9.3	VIGS treatment	The pTRV2-CsMORF9.3-silencing plasmid was constructed by inserting a 300 bp cDNA fragment of CsMORF9.3 into the pTRV2 viral vector	No mutant	The newly developed shoots and leaves exhibited a yellowing or pale-green phenotype; The chlorophyll fluorescence parameter Fv/Fm also showed a significant reduction	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	76.00%	High	Decreased	42159751

Camellia sinensis

CSS0008579

Chloroplast

ndhD

674

CDS

TPIA (https://tpia.teaplants.cn)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	pTRV2	Infected with pTRV1+pTRV2 Agrobacterium	NA	No mutant	Normal	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	94.00%	High	None	42159751
NA	pTRV2-CsMORF9.3	VIGS treatment	The pTRV2-CsMORF9.3-silencing plasmid was constructed by inserting a 300 bp cDNA fragment of CsMORF9.3 into the pTRV2 viral vector	No mutant	The newly developed shoots and leaves exhibited a yellowing or pale-green phenotype; The chlorophyll fluorescence parameter Fv/Fm also showed a significant reduction	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	78.00%	High	Decreased	42159751

Camellia sinensis

CSS0008579

Chloroplast

rpoA

200

CDS

TPIA (https://tpia.teaplants.cn)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	pTRV2	Infected with pTRV1+pTRV2 Agrobacterium	NA	No mutant	Normal	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	52.00%	Medium	None	42159751
NA	pTRV2-CsMORF9.3	VIGS treatment	The pTRV2-CsMORF9.3-silencing plasmid was constructed by inserting a 300 bp cDNA fragment of CsMORF9.3 into the pTRV2 viral vector	No mutant	The newly developed shoots and leaves exhibited a yellowing or pale-green phenotype; The chlorophyll fluorescence parameter Fv/Fm also showed a significant reduction	Leaf	NA	The PCR products were sequenced by Tsingke Biotechnology Co.	89.00%	High	Increased	42159751

Editing Factor:	MORF9.3
Synonym:	MULTIPLE ORGANELLAR RNA EDITING FACTOR 9; RIP9
Description:	Suppressing CsMORF9.3 expression reduces chlorophyll content, downregulates genes involved in chlorophyll biosynthesis and chloroplast development, as well as disrupts chloroplast RNA editing
Protein Family:	Multiple organellar RNA editin
Subclass:	NA
Physical Interaction:	CsMORFs; CsCRR21; CsCRR28; CsOTP84; CsLPA66; CsCHLD
Construct Structure:	MORF
Gene ID & Species:	CSS0008579 (Camellia sinensis)
Edited Gene(s):	matK rpoA ndhD
Editing Type(s):	C-to-U (8)
Publication(s):	[1] The function of tea plant CsMORF9.3 in chlorophyll biosynthesis and RNA editing, Plant Cell Reports, 2026. [PMID=42159751]

Summary

Editing Details