OTP72 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: OTP72
Synonym: ORGANELLE TRANSCRIPT PROCESSING 72
Description: Encodes a pentatricopeptide repeat (PPR) protein involved in RNA editing in mitochondria
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: At3g13880 (Arabidopsis thaliana)
Edited Gene(s): rpl16
Editing Type(s): C-to-U (4)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] The E domains of pentatricopeptide repeat proteins from different organelles are not functionally equivalent for RNA editing, Plant J, 2013. [PMID=23521509]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At3g13880 Mitochondrion rpl16 null CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentNormal growthLeaf15 daysPoisoned primer extension (PPE) assay100.00%CompleteNone23521509
SALK_040554otp72-1T-DNA insertionInsertion site in chromosome 34573006HomozygousNormal growthLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
SAIL_080885otp72-2T-DNA insertionInsertion site in chromosome 34572523HomozygousSmaller than wild‐type plantsLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
Arabidopsis thaliana At3g13880 Mitochondrion rpl16 440 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.55%HighNone32098170
Last update: Jul 2021 (version 1.0)