OTP86 - Plant Editosome Database - BIG Data Center

OTP86 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	OTP86
Synonym:	ORGANELLE TRANSCRIPT PROCESSING 86
Description:	Encodes a chloroplast RNA editing factor
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-DYW
Gene ID & Species:	At3g63370 (Arabidopsis thaliana)
Edited Gene(s):	rps14
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites, Plant Cell, 2009. [PMID=19934379]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

At3g63370

Chloroplast

null

CDS

C-to-U

NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_102445	Complement	Complement	The 2883 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB2 binary vectors and introduced into otp mutants via Agroba	Homozygous	NA	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	Restored	19934379
Col-0	Col-0	No treatment	No treatment	No treatment	Normal growth	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	None	19934379
SALK_102445	otp86	T-DNA insertion	The T-DNA insertion is at +698 in CDS	Homozygous	Normal growth	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	0.00%	Unedited	Absent	19934379

Arabidopsis thaliana

At3g63370

Plastid
Chloroplast

80

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	96.71%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	91.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	90.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	91.00%	High	Similar	28761003

Last update: Jul 2021 (version 1.0)