OTP86 - Plant Editosome Database

Editing Factor:	OTP86
Synonym:	ORGANELLE TRANSCRIPT PROCESSING 86
Description:	OTP80, OTP81, OTP85, and OTP86 target only one editing site each, OTP82 two sites, and OTP84 three sites in different transcripts; An analysis of the target sites requiring the five editing factors involved in editing of multiple sites (CRR22, CRR28, CLB19, OTP82, and OTP84) suggests that editing factors can generally distinguish pyrimidines from purines and, at some positions, must be able to recognize specific bases
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT3G63370 (Arabidopsis thaliana)
Edited Gene(s):	rps14
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites., The Plant cell, 2009. [PMID=19934379]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G63370

Plastid
Chloroplast

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	96.71%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	91.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	90.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	91.00%	High	Similar	28761003

Arabidopsis thaliana

AT3G63370

Chloroplast

CDS

Arabidopsis Genome Initiative

AP000423

Genomic position

37,161

C-to-U

NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_102445	Complement	Complement	The 2883 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB2 binary vectors and introduced into otp mutants via Agroba	Homozygous	NA	NA	NA	RT-PCR products are sequenced	NA	NA	Restored	19934379
Col-0	Col-0	Control	Control	Control	Normal growth	NA	NA	RT-PCR products are sequenced	NA	NA	None	19934379
SALK_102445	otp86	T-DNA insertion	The T-DNA insertion is at +698 in CDS	Homozygous	Normal growth	NA	NA	RT-PCR products are sequenced	0.00%	Unedited	Absent	19934379