Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| ZH11 | WT | Control | Control | No mutant | Normal | NA | NA | Real-time fluorescent quantitative PCR; BioEdit | 90.00% | High | None | 38549701 |
| NA | pgl3a | NA | A mutant library derived from Zhonghua 11 (ZH11); a japonica rice variety | NA | Pale green leaves and stunted growth | NA | NA | Real-time fluorescent quantitative PCR; BioEdit | 50.00% | Medium | Decreased | 38549701 |
| NA | cr-pgl3a-1 | A CRISPR-mediated mutant | Two targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targe | Homozygous; Knockout | A pale green leaf phenotype with decreased chlorophyll content | NA | NA | Real-time fluorescent quantitative PCR; BioEdit | 30.00% | Low | Decreased | 38549701 |
| NA | cr-pgl3a-2 | A CRISPR-mediated mutant | Two targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targe | Homozygous; Knockout | A pale green leaf phenotype with decreased chlorophyll content | NA | NA | Real-time fluorescent quantitative PCR; BioEdit | 40.00% | Medium | Decreased | 38549701 |