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PGL3A - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: PGL3A
Synonym: OsPGL3A
Description: OsPGL3A encodes a DYW-type pentatricopeptide repeat protein involved in chloroplast RNA processing and regulated chloroplast development
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: Os03g0136700 (Oryza sativa)
Edited Gene(s): rps8    rpoC2
Editing Type(s): C-to-U (8)
Publication(s): [1] encodes a DYW-type pentatricopeptide repeat protein involved in chloroplast RNA processing and regulated chloroplast development., Molecular breeding : new strategies in plant improvement, 2024. [PMID=38549701]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa Os03g0136700 Chloroplast rpoC2 4106 CDS IRGSP NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZH11WTControlControlNo mutantNormalNANAReal-time fluorescent quantitative PCR; BioEdit80.00%HighNone38549701
NApgl3aNAA mutant library derived from Zhonghua 11 (ZH11); a japonica rice varietyNAPale green leaves and stunted growthNANAReal-time fluorescent quantitative PCR; BioEdit50.00%MediumDecreased38549701
NAcr-pgl3a-1A CRISPR-mediated mutantTwo targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targeHomozygous; KnockoutA pale green leaf phenotype with decreased chlorophyll contentNANAReal-time fluorescent quantitative PCR; BioEdit20.00%LowDecreased38549701
NAcr-pgl3a-2A CRISPR-mediated mutantTwo targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targeHomozygous; KnockoutA pale green leaf phenotype with decreased chlorophyll contentNANAReal-time fluorescent quantitative PCR; BioEdit30.00%LowSimilar38549701
Oryza sativa Os03g0136700 Chloroplast rps8 182 CDS IRGSP NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZH11WTControlControlNo mutantNormalNANAReal-time fluorescent quantitative PCR; BioEdit90.00%HighNone38549701
NApgl3aNAA mutant library derived from Zhonghua 11 (ZH11); a japonica rice varietyNAPale green leaves and stunted growthNANAReal-time fluorescent quantitative PCR; BioEdit50.00%MediumDecreased38549701
NAcr-pgl3a-1A CRISPR-mediated mutantTwo targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targeHomozygous; KnockoutA pale green leaf phenotype with decreased chlorophyll contentNANAReal-time fluorescent quantitative PCR; BioEdit30.00%LowDecreased38549701
NAcr-pgl3a-2A CRISPR-mediated mutantTwo targeted sequences within the exon region of OsPGL3A were designed; The target site sequences were cloned into a single guide RNA expression vector pYLgRNA and subsequently inserted into the targeHomozygous; KnockoutA pale green leaf phenotype with decreased chlorophyll contentNANAReal-time fluorescent quantitative PCR; BioEdit40.00%MediumDecreased38549701
Last update: Feb 2026 (version 2.0)