PPR2263 - Plant Editosome Database

Editing Factor:	PPR2263
Synonym:	Pentatricopeptide Repeat Protein 2263
Description:	The maize (Zea mays) nuclear gene Pentatricopeptide repeat 2263 (PPR2263) encoding a DYW domain-containing PPR protein is required for RNA editing in the mitochondrial NADH dehydrogenase5 (nad5) and cytochrome b (cob) transcripts at the nad5-1550 and cob-908 sites, respectively; Its putative ortholog, MITOCHONDRIAL EDITING FACTOR29, fulfills the same role in Arabidopsis thaliana; Whereas mutant chloroplasts do not have major defects, mutant mitochondria lack complex III and are characterized by a compromised ultrastructure, increased transcript levels, and the induction of alternative oxidase
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AC215198.3_FG002 (Zea mays)
Edited Gene(s):	nad5 cob
Editing Type(s):	C-to-U (4)
Publication(s):	[1] PPR2263, a DYW-Subgroup Pentatricopeptide Repeat Protein, Is Required for Mitochondrial nad5 and cob Transcript Editing, Mitochondrion Biogenesis, and Maize Growth, The Plant Cell, 2012. [doi:10.1105/tpc.111.091074]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

AC215198.3_FG002

Mitochondrion

908

CDS

Maize Sequence database

DQ490952.1

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Mutator-induced Biogemma mutant collection	ppr2263	Mutator insertion	Mutator insertion leads to a truncated protein lacking three of its 13 PPR repeats as well as the C-terminal E/E+ and DYW domains	Homozygous	Small kernels and developmental delay	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22319053
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22319053