PPR596 - Plant Editosome Database

Editing Factor:	PPR596
Synonym:	PENTATRICOPEPTIDE REPEAT 596
Description:	Encoding a P-subfamily protein that influenced editing efficiency; The efficient excision of the third intron from the nad2 pre-mRNA; Insufficient splicing of this intron in ppr596 impairs respiratory-chain complex I biogenesis at the step of the insertion of the Nad2 subunit
Protein Family:	PPR
Subclass:	P
Construct Structure:	P
Gene ID & Species:	AT1G80270 (Arabidopsis thaliana)
Edited Gene(s):	rps3
Editing Type(s):	C-to-U (11)
Publication(s):	[1] PPR596 Is Required for nad2 mRNA Splicing and Complex I Biogenesis in Mitochondria of Arabidopsis thaliana., Physiologia plantarum, 2025. [PMID=40925877] [2] The involvement of a PPR protein of the P subfamily in partial RNA editing of an Arabidopsis mitochondrial transcript., Gene, 2010. [PMID=20117193]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G80270

Mitochondrion

1344

CDS

C-to-U

AGC=>AGU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_367_A06	complemented ppr596 mutant	Complementation	Morphological characters are recovered	NA	NA	Bud	NA	Direct Sequencing of PCR Products	35.00%	Low	Restored	20117193
SAIL_367_A06	complemented ppr596 mutant	Complementation	Morphological characters are recovered	NA	NA	Leaf	NA	Direct Sequencing of PCR Products	NA	Medium	Restored	20117193
SAIL_367_A06	mutant ppr596	T-DNA Insertion	T-DNA is inserted at 207 nt upstream of the stop codon of PPR596	Knockout; Homozygous	ppr596 mutants show delayed growth and flowering, and obvious morphological alterations such as curled leaves	Bud	NA	Direct Sequencing of PCR Products	65.00%	High	Increased	20117193
SAIL_367_A06	mutant ppr596	T-DNA Insertion	T-DNA is inserted at 207 nt upstream of the stop codon of PPR596	Knockout; Homozygous	ppr596 mutants show delayed growth and flowering, and obvious morphological alterations such as curled leaves	Leaf	NA	Direct Sequencing of PCR Products	NA	High	Increased	20117193
NA	WT	Control	Control	Control	Normal	Bud	NA	Direct Sequencing of PCR Products	30.00%	Low	None	20117193
NA	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	NA	Medium	None	20117193

Arabidopsis thaliana

AT1G80270

Mitochondrion

CDS

C-to-U

AGC=>AGU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Control	Control	No mutant	Normal	Seedling	NA	Quantitative RT-PCR and Sanger sequencing	36.00%	Low	None	40925877
SAIL_367_A06	ppr596	T-DNA insertion	The ppr596 mutant was isolated from T-DNA insertion line SAIL_367_A06 obtained from the European Arabidopsis Stock Centre	NA	The ppr596 mutant germinated and developed with a major delay and displayed a twisted-leaf phenotype	Seedling	NA	Quantitative RT-PCR and Sanger sequencing	60.00%	High	Increased	40925877
NA	Comp1	Complementation	A 3.7-kb genomic fragment comprising the PPR596 gene was PCR-amplified with Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific Inc.), and ligated into the pGEM-T Easy vector (Promega GmbH)	NA	Restored plant growth to being wild type-like	Seedling	NA	Quantitative RT-PCR and Sanger sequencing	30.00%	Low	Restored	40925877
NA	ndufv1	Mutant	NA	NA	NA	Seedling	NA	Quantitative RT-PCR and Sanger sequencing	41.00%	Medium	Similar	40925877
NA	rug3	Mutant	NA	NA	NA	Seedling	NA	Quantitative RT-PCR and Sanger sequencing	42.00%	Medium	Similar	40925877