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PPR596 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: PPR596
Synonym: PENTATRICOPEPTIDE REPEAT 596
Description: Encoding a P-subfamily protein that influenced editing efficiency; The efficient excision of the third intron from the nad2 pre-mRNA; Insufficient splicing of this intron in ppr596 impairs respiratory-chain complex I biogenesis at the step of the insertion of the Nad2 subunit
Protein Family: PPR
Subclass: P
Physical Interaction: NA
Construct Structure: P
Gene ID & Species: AT1G80270 (Arabidopsis thaliana)
Edited Gene(s): rps3
Editing Type(s): C-to-U (11)
Publication(s): [1] PPR596 Is Required for nad2 mRNA Splicing and Complex I Biogenesis in Mitochondria of Arabidopsis thaliana., Physiologia plantarum, 2025. [PMID=40925877]
[2] The involvement of a PPR protein of the P subfamily in partial RNA editing of an Arabidopsis mitochondrial transcript., Gene, 2010. [PMID=20117193]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G80270 Mitochondrion rps3 1344 CDS NA NA NA NA C-to-U AGC=>AGU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SAIL_367_A06complemented ppr596 mutantComplementationMorphological characters are recoveredNANABudNADirect Sequencing of PCR Products35.00%LowRestored20117193
SAIL_367_A06complemented ppr596 mutantComplementationMorphological characters are recoveredNANALeafNADirect Sequencing of PCR ProductsNAMediumRestored20117193
SAIL_367_A06mutant ppr596T-DNA InsertionT-DNA is inserted at 207 nt upstream of the stop codon of PPR596Knockout; Homozygousppr596 mutants show delayed growth and flowering, and obvious morphological alterations such as curled leavesBudNADirect Sequencing of PCR Products65.00%HighIncreased20117193
SAIL_367_A06mutant ppr596T-DNA InsertionT-DNA is inserted at 207 nt upstream of the stop codon of PPR596Knockout; Homozygousppr596 mutants show delayed growth and flowering, and obvious morphological alterations such as curled leavesLeafNADirect Sequencing of PCR ProductsNAHighIncreased20117193
NAWTControlControlControlNormalBudNADirect Sequencing of PCR Products30.00%LowNone20117193
NAWTControlControlControlNormalLeafNADirect Sequencing of PCR ProductsNAMediumNone20117193
Arabidopsis thaliana AT1G80270 Mitochondrion rps3 NA CDS NA NC_037304.1 Genomic position 23091 C-to-U AGC=>AGU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)Col-0ControlControlNo mutantNormalSeedlingNAQuantitative RT-PCR and Sanger sequencing36.00%LowNone40925877
SAIL_367_A06ppr596T-DNA insertionThe ppr596 mutant was isolated from T-DNA insertion line SAIL_367_A06 obtained from the European Arabidopsis Stock CentreNAThe ppr596 mutant germinated and developed with a major delay and displayed a twisted-leaf phenotypeSeedlingNAQuantitative RT-PCR and Sanger sequencing60.00%HighIncreased40925877
NAComp1ComplementationA 3.7-kb genomic fragment comprising the PPR596 gene was PCR-amplified with Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific Inc.), and ligated into the pGEM-T Easy vector (Promega GmbH)NARestored plant growth to being wild type-likeSeedlingNAQuantitative RT-PCR and Sanger sequencing30.00%LowRestored40925877
NAndufv1MutantNANANASeedlingNAQuantitative RT-PCR and Sanger sequencing41.00%MediumSimilar40925877
NArug3MutantNANANASeedlingNAQuantitative RT-PCR and Sanger sequencing42.00%MediumSimilar40925877
Last update: Feb 2026 (version 2.0)