Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| GH moss line | GH line | Without β-estradiol | Control | No mutant | Normal | Protonema | 4 days | Direct Sequencing of PCR Products | 65.00% | High | None | 25277299 |
| GH moss line | GH line | With β-estradiol | Control | No mutant | Normal | Protonema | 4 days | Direct Sequencing of PCR Products | 65.00% | High | None | 25277299 |
| GH moss line | 45RNAi #3 | RNAi without β-estradiol | An inducible RNAi construct is introduced into the transgenic GH moss line | Knockdown | Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days | Protonema | 4 days | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 25277299 |
| GH moss line | 45RNAi #3 | RNAi with β-estradiol | An inducible RNAi construct is introduced into the transgenic GH moss line | Knockdown | Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days | Protonema | 4 days | Direct Sequencing of PCR Products | 16.70% | Poor | Decreased | 25277299 |
| GH moss line | 45RNAi #5 | RNAi without β-estradiol | An inducible RNAi construct is introduced into the transgenic GH moss line | Knockdown | Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days | Protonema | 4 days | Direct Sequencing of PCR Products | 40.00% | Medium | Decreased | 25277299 |
| GH moss line | 45RNAi #5 | RNAi with β-estradiol | An inducible RNAi construct is introduced into the transgenic GH moss line | Knockdown | Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days | Protonema | 4 days | Direct Sequencing of PCR Products | 2.00% | Poor | Decreased | 25277299 |
| NA | 45OX#6 | Overexpression | The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter | NA | The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type | Protonema | 4 days | Direct Sequencing of PCR Products | 100.00% | Complete | Decreased | 25277299 |
| NA | 45OX#203 | Overexpression | The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter | NA | The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type | Protonema | 4 days | Direct Sequencing of PCR Products | 90.00% | High | Decreased | 25277299 |
| NA | 45OX#114 | Overexpression | The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter | NA | The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type | Protonema | 4 days | Direct Sequencing of PCR Products | 90.00% | High | Decreased | 25277299 |
| NA | CT | Transformation | The empty pOX7WH1 is introduced | NA | The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type | Protonema | 4 days | Direct Sequencing of PCR Products | 68.00% | High | None | 25277299 |
| NA | WT | Control | Control | No mutant | Normal | Protonema | 4 days | Direct Sequencing of PCR Products | 65.00% | High | None | 25277299 |
