PED
Plant
Editosome
Database
PpPPR_78 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: PpPPR_78
Synonym: Phypa_192620
Description: Encoding a DYW-type PPR protein responsible for mitochondrial editing
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW, PLS-E/E+-DYW, PLS-E-DYW
Gene ID & Species: LOC112275185 (Physcomitrium patens)
Edited Gene(s): cox1    rps14
Editing Type(s): C-to-U (58)
Publication(s): [1] The L motifs of two moss pentatricopeptide repeat proteins are involved in RNA editing but predominantly not in RNA recognition., PloS one, 2020. [PMID=32348368]
[2] A Single-Target Mitochondrial RNA Editing Factor of Funaria hygrometrica Can Fully Reconstitute RNA Editing at Two Sites in Physcomitrella patens., Plant & cell physiology, 2017. [PMID=28394399]
[3] The PPR-DYW proteins are required for RNA editing of rps14, cox1 and nad5 transcripts in Physcomitrella patens mitochondria., FEBS letters, 2011. [PMID=21708151]
[4] Assigning DYW-type PPR proteins to RNA editing sites in the funariid mosses Physcomitrella patens and Funaria hygrometrica., The Plant journal : for cell and molecular biology, 2011. [PMID=21466601]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Physcomitrium patens LOC112275185 Mitochondrion cox1 755 CDS P. patens 3.0 genome assembly (www.cosmoss.org)
P. patens subsp. patens genome ver. 1.1
NA		 NC_007945
NA		 NA NA C-to-U UCG=>UUG
NA=>NA		 S=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Bruch & Schimp. strain Gransden 2004FhPPR_78_trunc 1ComplementationComplementation lines are introduce truncated versions of F. hygrometrica PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78_trunc 2ComplementationComplementation lines are introduce truncated versions of F. hygrometrica PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-1ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-2ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-3ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PPR_78 KONANAKnockoutNo discernible phenotypeProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004WTControlControlControlNormalProtonemaNADirect Sequencing of PCR Products100.00%CompleteNone28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 1ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products89.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 2ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 3ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 1ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 2ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 3ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 2ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products2.00%PoorDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 3ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products3.00%PoorDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 4ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products4.00%PoorDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 5ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products2.00%PoorDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-1ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products88.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-2ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-3ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products100.00%CompleteRestored28394399
NA△78Gene cassettehpt gene cassetteknockoutSlightly reduced growth of the protonemataProtonema3 daysDirect Sequencing of PCR Products0.00%UneditedAbsent21708151
NAWTControlControlControlNormalProtonema3 daysDirect Sequencing of PCR Products100.00%CompleteNone21708151
NAPhypa_192620 KOGene cassetteDisruption of the Phypa_192620 locus by homologous recombination introducing the nptII cassetteknockoutIndistinguishable from wild-typeProtonema2-4 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent21466601
NAWTControlControlControlNormalProtonema2-4 weeksDirect Sequencing of PCR Products100.00%CompleteNone21466601
NA78 comp #1Complemented with PpPPR_78 (78comp) #1PpPPR_78 KO mosses complemented with PpPPR_78 (78comp)KnockoutNAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi99.00%HighRestored32348368
NA78 comp #3Complemented with PpPPR_78 (78comp) #3PpPPR_78 KO mosses complemented with PpPPR_78 (78comp)KnockoutNAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi96.00%HighRestored32348368
NA78-16L19L #1Complemented with 78-16L19L #1Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi87.00%HighNA32348368
NA78-16L19L #2Complemented with 78-16L19L #2Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi94.00%HighRestored32348368
NA78-16L19L #3Complemented with 78-16L19L #3Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi92.00%HighRestored32348368
Physcomitrium patens LOC112275185 Mitochondrion rps14 137 CDS P. patens 3.0 genome assembly (www.cosmoss.org)
P. patens subsp. patens genome ver. 1.1
NA		 NC_007945
NA		 NA NA C-to-U UCG=>UUG
NA=>NA		 S=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Bruch & Schimp. strain Gransden 2004FhPPR_78_trunc 1ComplementationComplementation lines are introduce truncated versions of F. hygrometrica PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78_trunc 2ComplementationComplementation lines are introduce truncated versions of F. hygrometrica PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-1ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products88.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-2ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products89.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004FhPPR_78-3ComplementationComplementation lines are introduce the Funaria hygrometrica PPR_78 into the P. patens KO backgroundNANAProtonemaNADirect Sequencing of PCR Products92.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004PPR_78 KONANAKnockoutNo discernible phenotypeProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004WTControlControlControlNormalProtonemaNADirect Sequencing of PCR Products75%HighNone28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 1ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products79.00%HighDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 2ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products83.00%HighDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_DYW 3ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the DYW domain alone swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products70.00%HighDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 1ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products62.00%HighDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 2ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products63.00%HighDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_79_E-DYW 3ComplementationThe P. patens KO PPR_78 lines are complemented with PPR_78 with the E-DYW domains swapped with the one of PPR_79Showed high expression levels (a minimum of 100-fold)NAProtonemaNADirect Sequencing of PCR Products26.00%LowDecreased28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 2ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 3ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 4ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78_trunc 5ComplementationThe complementation lines are introduced truncated versions of P. patens PPR_78 lacking the DYW domain into the P. patens PPR_78 KO backgroundNANAProtonemaNADirect Sequencing of PCR Products0.00%UneditedAbsent28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-1ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products88.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-2ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products86.00%HighRestored28394399
Bruch & Schimp. strain Gransden 2004PpPPR_78-3ComplementationThe complete P. patens PPR_78 driven by the strong rice actin1 promoter is introduced into the KO_PPR_78 backgroundExpression levels of inserted genes were shown to be a minimum of 20-fold higherNAProtonemaNADirect Sequencing of PCR Products85.00%HighRestored28394399
NA△78Gene cassettehpt gene cassetteknockoutSlightly reduced growth of the protonemataProtonema3 daysDirect Sequencing of PCR Products0.00%UneditedAbsent21708151
NAWTControlControlControlNormalProtonema3 daysDirect Sequencing of PCR ProductsNAHighNone21708151
NAPhypa_192620 KOGene cassetteDisruption of the Phypa_192620 locus by homologous recombination introducing the nptII cassetteknockoutIndistinguishable from wild-typeProtonema2-4 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent21466601
NAWTControlControlControlNormalProtonema2-4 weeksDirect Sequencing of PCR ProductsNAHighNone21466601
NA78 comp #1Complemented with PpPPR_78 (78comp) #1PpPPR_78 KO mosses complemented with PpPPR_78 (78comp)KnockoutNAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi94.00%HighRestored32348368
NA78 comp #3Complemented with PpPPR_78 (78comp) #3PpPPR_78 KO mosses complemented with PpPPR_78 (78comp)KnockoutNAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi88.00%HighNA32348368
NA78-16L19L #1Complemented with 78-16L19L #1Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi18.00%PoorDecreased32348368
NA78-16L19L #2Complemented with 78-16L19L #2Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi34.00%LowDecreased32348368
NA78-16L19L #3Complemented with 78-16L19L #3Creating the 78-16L19L variant, wherein 16L[ID] and 19L[VT] were changed to 16L[ND] and 19L[TN] for the recognition of U and A, respectively. Introducing each of them into the PpPPR_78 KO mutant (Δ78–NANAProtonema4-day-oldRT-PCR, cDNA sequencing and chromatographs were analyzed usi29.00%LowDecreased32348368
Last update: Feb 2026 (version 2.0)