Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Recombinant of Col and Ler | Control | Not inoculated | NA | Non-mutant | Under UV light, the silenced plants show a characteristic red phenotype resulting from chlorophyll autofluorescence, while unsilenced plants fluoresce green. | Leaf | NA | Poisoned primer extension (PPE) assay | 53.00% | Medium | None | 20974892 |
| Recombinant of Col and Ler | Control | Empty vector | The empty vector control was built by first cutting the pCR8-GW-TOPO vector with EcoRI, which releases the amplicon, and then self-ligating the vector with a T4 DNA ligase (Fermentas). | Non-mutant | Under UV light, the silenced plants show a characteristic red phenotype resulting from chlorophyll autofluorescence, while unsilenced plants fluoresce green. | Leaf | NA | Poisoned primer extension (PPE) assay | 46.00% | Medium | None | 20974892 |
| Recombinant of Col and Ler | Silenced | Virus-induced gene silencing (VIGS) of REME1 | The silencing fragment for REME1 was amplified by PCR using the following gene sequence tag primers designed by the CATMA database (Crowe et al., 2003): REME1-F1 (5′-TGGATTCATTGCAAAGTCATGG-3′) and REM | NA | Under UV light, the silenced plants show a characteristic red phenotype resulting from chlorophyll autofluorescence, while unsilenced plants fluoresce green. | Leaf | NA | Poisoned primer extension (PPE) assay | 31.00% | Low | Decreased | 20974892 |
| Ler | WT | No treatment | No treatment | No treatment | Normal | Leaf | NA | Poisoned primer extension (PPE) assay | 17.00% | Poor | None | 20974892 |
| GT_5_23135 | GT_5_23135 | T-DNA insertion | The insertion was located at nucleotide position 1,025, corresponding to the middle of the fourth P repeat | Homozygous | The mutant plants show no detectable phenotypic defects and are absolutely indistinguishable from their wild-type siblings when grown in growth room conditions | Leaf | NA | Poisoned primer extension (PPE) assay | 6.00% | Poor | Decreased | 20974892 |
| Col | Col | No treatment | No treatment | No treatment | Normal | Leaf | NA | Poisoned primer extension (PPE) assay | 50.00% | Medium | None | 20974892 |
| Ler | Ler | No treatment | No treatment | No treatment | Normal | Leaf | NA | Poisoned primer extension (PPE) assay | 20.00% | Low | None | 20974892 |
| Ler | T0 | Transformation | A binary vector harboring the REME1 Col allele (high editor) under the control of a 35S promoter to transform the Ler accession (low editor). | NA | NA | Leaf | NA | Poisoned primer extension (PPE) assay | 45.00%-90. | NA | None | 20974892 |
| Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 50.57% | Medium | None | 32098170 |
| Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 22.00% | Low | None | 28761003 |
| Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 64.00% | High | Increased | 28761003 |
| Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 41.00% | Medium | Increased | 28761003 |