REME2 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: REME2
Synonym: REQUIRED FOR EFFICIENCY OF MITOCHONDRIAL EDITING 2
Description: Encodes a PPR-DYW protein required for editing of specific C targets in Arabidopsis mitochondria.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: AT4G15720 (Arabidopsis thaliana)
Edited Gene(s): rps3    rps4
Editing Type(s): C-to-U (20)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Quantitative trait locus mapping identifies REME2, a PPR-DYW protein required for editing of specific C targets in Arabidopsis mitochondria, RNA Biology, 2013. [PMID=23807026]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G15720 Mitochondrion rps3 1534 CDS C-to-U CGU=>UGU
NA=>NA
R=>C
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay72.00%HighNone23807026
LerWTNo treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay90.00%HighNone23807026
SALK_151755T-DNA mutant (-/-)T-DNA InsertionT-DNA is located at 85 nucleotides from the stop codon, in the 3'UTRHomozygous; KnockdownNANANAPoisoned Primer Extension (PPE) Assay63.00%HighDecreased23807026
NAWT (Control of T-DNA mutant)No treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay86.00%HighNone23807026
NASilencing of REME2 by VIGSVirus-induced gene silencing (VIGS)NANANANANAPoisoned Primer Extension (PPE) Assay49.00%MediumDecreased23807026
NAWT (Control of VIGS)No treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay77.00%HighNone23807026
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq64.98%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract80.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract64.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract62.00%HighDecreased28761003
Arabidopsis thaliana AT4G15720 Mitochondrion rps4 175 CDS C-to-U CCG=>UCG
NA=>NA
P=>S
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay41.00%MediumNone23807026
LerWTNo treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay77.00%HighNone23807026
SALK_151755T-DNA mutant (-/-)T-DNA InsertionT-DNA is located at 85 nucleotides from the stop codon, in the 4'UTRHomozygous; KnockdownNANANAPoisoned Primer Extension (PPE) Assay42.00%MediumDecreased23807026
NAWT (Control of T-DNA mutant)No treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay68.00%HighNone23807026
NASilencing of REME2 by VIGSVirus-induced gene silencing (VIGS)NANANANANAPoisoned Primer Extension (PPE) Assay31.00%LowDecreased23807026
NAWT (Control of VIGS)No treatmentNo treatmentNo treatmentNo treatmentNANAPoisoned Primer Extension (PPE) Assay47.00%MediumNone23807026
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq74.26%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)