REME2 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: REME2
Synonym: REQUIRED FOR EFFICIENCY OF MITOCHONDRIAL EDITING 2
Description: Encoding a PPR-DYW protein required for editing of specific C targets in Arabidopsis mitochondria.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: AT4G15720 (Arabidopsis thaliana)
Edited Gene(s): rps3    rps4
Editing Type(s): C-to-U (20)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Quantitative trait locus mapping identifies REME2, a PPR-DYW protein required for editing of specific C targets in Arabidopsis mitochondria., RNA biology, 2013. [PMID=23807026]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G15720 Mitochondrion rps3 1534 CDS C-to-U CGU=>UGU
NA=>NA
R=>C
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay72.00%HighNone23807026
LerWTControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay90.00%HighNone23807026
SALK_151755T-DNA mutant (-/-)T-DNA InsertionT-DNA is located at 85 nucleotides from the stop codon, in the 3'UTRHomozygous; KnockdownNANANAPoisoned Primer Extension (PPE) Assay63.00%HighDecreased23807026
NAWT (Control of T-DNA mutant)ControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay86.00%HighNone23807026
NASilencing of REME2 by VIGSVirus-induced gene silencing (VIGS)NANANANANAPoisoned Primer Extension (PPE) Assay49.00%MediumDecreased23807026
NAWT (Control of VIGS)ControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay77.00%HighNone23807026
Col-0WTControlControlControlNANANARNA-seq64.98%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract80.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract64.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract62.00%HighDecreased28761003
Arabidopsis thaliana AT4G15720 Mitochondrion rps4 175 CDS C-to-U CCG=>UCG
NA=>NA
P=>S
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay41.00%MediumNone23807026
LerWTControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay77.00%HighNone23807026
SALK_151755T-DNA mutant (-/-)T-DNA InsertionT-DNA is located at 85 nucleotides from the stop codon, in the 4'UTRHomozygous; KnockdownNANANAPoisoned Primer Extension (PPE) Assay42.00%MediumDecreased23807026
NAWT (Control of T-DNA mutant)ControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay68.00%HighNone23807026
NASilencing of REME2 by VIGSVirus-induced gene silencing (VIGS)NANANANANAPoisoned Primer Extension (PPE) Assay31.00%LowDecreased23807026
NAWT (Control of VIGS)ControlControlControlNormalNANAPoisoned Primer Extension (PPE) Assay47.00%MediumNone23807026
Col-0WTControlControlControlNANANARNA-seq74.26%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)