RF1A - Plant Editosome Database - CNCB-NGDC

RF1A - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	RF1A
Synonym:	NA
Description:	RF1A and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage (RF1A) or degradation (RF1B) of dicistronic B-atp6/orf79 mRNA; In the presence of both restorers, RF1A was epistatic over RF1B in the mRNA processing; RF1A plays an additional role in promoting the editing of atp6 mRNAs, independent of its cleavage function
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	P
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	LOC_Os10g35440 (Oryza sativa)
Edited Gene(s):	atp6
Editing Type(s):	C-to-U (9)
Publication(s):	[1] Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing, The Plant Cell, 2006. [doi:10.1105/tpc.105.038240]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os10g35440

Mitochondrion

13

CDS

NA

NA

NA

NA

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
CMS-BT (KFA)	CMS-BT (KFA)	NA	NA	NA	Male-sterility phenotype appears in male gametophytes	Panicle	NA	Direct Sequencing of PCR Products	43.60%	Medium	None	16489123
CMS-BT (KFA)	CMS-BT (KFA)	NA	NA	NA	Male-sterility phenotype appears in male gametophytes	Panicle	NA	Direct Sequencing of PCR Products	44.40%	Medium	None	16489123
CMS-BT (KFA)	Rf1a-T1	Transformation	Rf1A is introduced by transformation	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	69.70%	High	Increased	16489123
CMS-BT (KFA)	Rf1a-T1	Transformation	Rf1A is introduced by transformation	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	90.30%	High	Increased	16489123
CMS-BT (KFA)	Rf1a-T2	Transformation	Rf1A is introduced by transformation	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	45.20%	Medium	Similar	16489123
CMS-BT (KFA)	Rf1a-T2	Transformation	Rf1A is introduced by transformation	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	43.30%	Medium	Similar	16489123
CMS-BT (KFA)	Hyb	Transformation	Rf1A is introduced by crossing	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	65.20%	High	Increased	16489123
CMS-BT (KFA)	Hyb	Transformation	Rf1A is introduced by crossing	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	82.30%	High	Increased	16489123
CMS-BT (KFA)	maintainer line (KFB)	NA	NA	NA	NA	Panicle	NA	Direct Sequencing of PCR Products	48.50%	Medium	None	16489123

Last update: Sep 2026 (version 2.0)