RF1A - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: RF1A
Synonym: NA
Description: Encodes a protein that plays an additional role in promoting the editing of atp6 mRNAs, independent of its cleavage function.
Protein Family: PPR
Subclass: P
Construct Structure: NA
Gene ID & Species: LOC_Os10g35440 (Oryza sativa)
Edited Gene(s): B-atp6    N-atp6
Editing Type(s): C-to-U (9)
Publication(s): [1] Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing, The Plant Cell, 2006. [PMID=16489123]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_Os10g35440 Mitochondrion B-atp6 13 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
CMS-BT (KFA)CMS-BT (KFA)NANANAMale-sterility phenotype appears in male gametophytesPanicleNADirect Sequencing of PCR Products43.60%MediumNone16489123
CMS-BT (KFA)Rf1a-T1TransformationRf1A is introduced by transformationNANAPanicleNADirect Sequencing of PCR Products69.70%HighIncreased16489123
CMS-BT (KFA)Rf1a-T2TransformationRf1A is introduced by transformationNANAPanicleNADirect Sequencing of PCR Products45.20%MediumSimilar16489123
CMS-BT (KFA)HybTransformationRf1A is introduced by crossingNANAPanicleNADirect Sequencing of PCR Products65.20%HighIncreased16489123
Oryza sativa LOC_Os10g35440 Mitochondrion N-atp6 13 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
CMS-BT (KFA)CMS-BT (KFA)NANANAMale-sterility phenotype appears in male gametophytesPanicleNADirect Sequencing of PCR Products44.40%MediumNone16489123
CMS-BT (KFA)Rf1a-T1TransformationRf1A is introduced by transformationNANAPanicleNADirect Sequencing of PCR Products90.30%HighIncreased16489123
CMS-BT (KFA)Rf1a-T2TransformationRf1A is introduced by transformationNANAPanicleNADirect Sequencing of PCR Products43.30%MediumSimilar16489123
CMS-BT (KFA)HybTransformationRf1A is introduced by crossingNANAPanicleNADirect Sequencing of PCR Products82.30%HighIncreased16489123
CMS-BT (KFA)maintainer line (KFB)NANANANAPanicleNADirect Sequencing of PCR Products48.50%MediumNone16489123
Last update: Jul 2021 (version 1.0)