SMK4 - Plant Editosome Database

Summary

Editing Factor:	SMK4
Synonym:	SMALL KERNEL4
Description:	Null mutation of Smk4 slows plant growth and development, causing small plants, delayed flowering time, and small kernels; Loss of Smk4 function abolishes C-to-U editing at position 1489 of the cytochrome c oxidase1 (cox1) transcript, causing an amino acid change from serine to proline at 497 in Cox1; Cox1 is a core component of mitochondrial complex IV. Indeed, complex IV activity is reduced in the smk4, along with drastically elevated expression of alternative oxidases (AOX)
Protein Family:	PPR
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2
Gene ID & Species:	GRMZM2G459532 (Zea mays)
Edited Gene(s):	cox1
Editing Type(s):	C-to-U (16)
Publication(s):	[1] SMALL KERNEL4 is required for mitochondrial cox1 transcript editing and seed development in maize., Journal of integrative plant biology, 2020. [PMID=31332949]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G459532

Mitochondrion

cox1

1489

CDS

Maizegdb database (www.maizegdb.org)

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT	Control	Control	None	Grow well	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	High	None	31332949
W22	smk4-1	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459532 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-2	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459533 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Low	NA	31332949
W22	smk4-3	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459534 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	WT	Control	Control	None	Grow well	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Medium	None	31332949
W22	smk4-1	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459532 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-2	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459533 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-3	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459534 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	WT	Control	Control	None	Grow well	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	High	None	31332949
W22	smk4-1	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459532 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-2	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459533 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Low	NA	31332949
W22	smk4-3	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459534 gene	Null allele	Slow plant growth and development,	Seed	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	WT	Control	Control	None	Grow well	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Medium	None	31332949
W22	smk4-1	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459532 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-2	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459533 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949
W22	smk4-3	Mutation	A Mu insertion linked to the smk4-1 mutation was identified at 45 bp downstream from the ATG in the GRMZM2G459534 gene	Null allele	Slow plant growth and development,	Leaf	12 DAP	Direct Sequencing of RT-PCR Products	NA	Unedited	NA	31332949