SST1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Glycine max

SoyZH13_09G000500

Mitochondrion

atp6-1

Gmax_ZH13

cDNA

167

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W82	WT	Control	Control	No mutant	Normal	Root	NA	RT-PCR products were directly sequenced	38.00%	Low	None	40995854
W82	WT	100mM NaCl	100mM NaCl	No mutant	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	37.00%	Low	None	40995854
W82	sst1-1	Deletion	A 1-bp deletion in the coding region	Knockout	Under control conditions, there were no significant differences in the fresh weight of leaves and roots, the Na+ and K+ contents, Na+/K+ ratio in leaves and roots, or the H2O2 content in roots between the wild-type (WT) and sst1 mutants, but the plant height in sst1 mutants was significantly greater than in WT	Root	NA	RT-PCR products were directly sequenced	9.00%	Poor	Decreased	40995854
W82	sst1-1	Deletion; 100mM NaCl	A 1-bp deletion in the coding region; 100mM NaCl	Knockout	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	22.00%	Low	Decreased	40995854
W82	sst1-1 comp	Complementation; 100mM NaCl	NA	NA	The morphology and internal structure of root tip mitochondria in complementary soybean lines were restored to WT levels under both control and salt stress conditions	Root	NA	RT-PCR products were directly sequenced	40.00%	Medium	Restored	40995854

Glycine max

SoyZH13_09G000500

Mitochondrion

cob

Gmax_ZH13

cDNA

113

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W82	WT	Control	Control	No mutant	Normal	Root	NA	RT-PCR products were directly sequenced	64.00%	High	None	40995854
W82	WT	100mM NaCl	100mM NaCl	No mutant	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	53.00%	Medium	None	40995854
W82	sst1-1	Deletion	A 1-bp deletion in the coding region	Knockout	Under control conditions, there were no significant differences in the fresh weight of leaves and roots, the Na+ and K+ contents, Na+/K+ ratio in leaves and roots, or the H2O2 content in roots between the wild-type (WT) and sst1 mutants, but the plant height in sst1 mutants was significantly greater than in WT	Root	NA	RT-PCR products were directly sequenced	26.00%	Low	Decreased	40995854
W82	sst1-1	Deletion; 100mM NaCl	A 1-bp deletion in the coding region; 100mM NaCl	Knockout	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	21.00%	Low	Decreased	40995854
W82	sst1-1 comp	Complementation; 100mM NaCl	NA	NA	The morphology and internal structure of root tip mitochondria in complementary soybean lines were restored to WT levels under both control and salt stress conditions	Root	NA	RT-PCR products were directly sequenced	47.00%	Medium	Restored	40995854

Glycine max

SoyZH13_09G000500

Mitochondrion

nad3

Gmax_ZH13

cDNA

230

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W82	WT	Control	Control	No mutant	Normal	Root	NA	RT-PCR products were directly sequenced	54.00%	Medium	None	40995854
W82	WT	100mM NaCl	100mM NaCl	No mutant	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	50.00%	Medium	None	40995854
W82	sst1-1	Deletion	A 1-bp deletion in the coding region	Knockout	Under control conditions, there were no significant differences in the fresh weight of leaves and roots, the Na+ and K+ contents, Na+/K+ ratio in leaves and roots, or the H2O2 content in roots between the wild-type (WT) and sst1 mutants, but the plant height in sst1 mutants was significantly greater than in WT	Root	NA	RT-PCR products were directly sequenced	31.00%	Low	Decreased	40995854
W82	sst1-1	Deletion; 100mM NaCl	A 1-bp deletion in the coding region; 100mM NaCl	Knockout	Under 100 mM NaCl conditions, both sst1-1 and sst1-2 presented significantly increased salt tolerance compared with the WT, with substantially increased SILS; The fresh leaf weight and fresh root weight of the sst1-1 and sst1-2 mutants were comparable to those of the WT; The sst1 mutants exhibited distinct Na+ and K+ accumulation patterns: while leaf Na+ content increased, root Na+ remained unchanged; Leaf K+ content rose, whereas root K+ declined; The leaf Na+/K+ ratio in mutants was significantly lower than in WT, while the root Na+/K+ ratio showed a significant increase compared to WT roots	Root	NA	RT-PCR products were directly sequenced	24.00%	Low	Decreased	40995854
W82	sst1-1 comp	Complementation; 100mM NaCl	NA	NA	The morphology and internal structure of root tip mitochondria in complementary soybean lines were restored to WT levels under both control and salt stress conditions	Root	NA	RT-PCR products were directly sequenced	33.00%	Low	Decreased	40995854

Editing Factor:	SST1
Synonym:	Soybean Salt Tolerant 1
Description:	Mitochondrial morphology and H2O2 homeostasis in root cells
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	GmCHX1; GmMORF1b
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	SoyZH13_09G000500 (Glycine max)
Edited Gene(s):	cob nad3 atp6-1
Editing Type(s):	C-to-U (15)
Publication(s):	[1] Natural Variation of a PPR Coding Gene SST1 Confers Salt Tolerance During Soybean Domestication, Plant Biotechnology Journal, 2025. [doi:10.1111/pbi.70382]

Summary

Editing Details