Plant Editosome Database
a curated database of RNA editosome in plants

WSL4 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: WSL4
Synonym: WHITE STRIPE LEAF4
Description: The wsl4 mutant develops white-striped leaves during early leaf development, characterized by decreased chlorophyll content and malformed chloroplasts; Notably, the wsl4 mutant causes defects in the splicing of atpF, ndhA, rpl2, and rps12; Findings identify WSL4 as a novel P-family PPR protein essential for chloroplast RNA group II intron splicing during early leaf development in rice
Protein Family: PPR
Subclass: P
Construct Structure: P
Gene ID & Species: LOC_Os02g35750 (Oryza sativa)
Edited Gene(s): rpoB
Editing Type(s): C-to-U (8)
Publication(s): [1] Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development., Frontiers in plant science, 2017. [PMID=28694820]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_Os02g35750 Chloroplast rpoB 545 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
RX69WTWild TypeWild TypeNo mutantNormalLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced15.00%PoorNone28694820
RX69wsl4The wsl4 mutant was isolated from a 60Co-irradiated mutant pool of japonica cultivar RX69.NARecessive; HomozygousThe leaves of seedlings at the 3-4 leaf stage exhibited a bleached appearance with the most extreme symptoms observed in the third leaf and distal half of the fourth leaf. All leaves after the 5-leaf stage were as green as WT plants. Young leaves on newly emerging tillers of the wsl4 mutant also exhibited the green/white leaf color defect. No difference was observed between WT and wsl4 mutant after the heading. Consistent with these observations, levels of Chl a and b in striped leaves of wsl4 plants were much lower than those in WT. Compared with WT plants, wsl4 mutants showed no statistically significant differences in plant height, number of tillers, number of branches per panicle, number of spikelets per panicle, and 1000-grain weight. In contrast to the moderate striped phenotype and Chl accumulations under optimum temperature (L30/D25), the wsl4 mutant exhibited less color deficiency and accumulated much higher Chl contents when grown at a constant 30°C (C30). At a constant 20°C the wsl4 mutant showed more extreme symptoms and Chl content that were barely detectable.LeafThe third leaf at the three-leaf stageRT-PCR products were sequenced75.00%HighIncreased28694820
RX69ComComplementationFor complementation of the wsl4 mutation a 5.7 kb WT genomic fragment containing a 2.2 kb upstream sequence, the entire coding region of WSL4, and a 2.0 kb downstream sequence was amplified from RX69 Recessive; HomozygousGreen leafLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced15.00%PoorRestored28694820
RX69RNAiRNA interferenceFor RNAi test, the construct pCUbi1390-DFAD2 (ubiquitin promoter and a FAD2 intron inserted into pCAMBIA1390) was used as an RNAi vector (Stoutjesdijk et al., 2002; Wu et al., 2007). Both anti-sense aNAWhite leafLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced75.00%HighIncreased28694820
Oryza sativa LOC_Os02g35750 Chloroplast rpoB 560 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
RX69WTWild TypeWild TypeNo mutantNormalLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced20.00%LowNone28694820
RX69wsl4The wsl4 mutant was isolated from a 60Co-irradiated mutant pool of japonica cultivar RX69.NARecessive; HomozygousThe leaves of seedlings at the 3-4 leaf stage exhibited a bleached appearance with the most extreme symptoms observed in the third leaf and distal half of the fourth leaf. All leaves after the 5-leaf stage were as green as WT plants. Young leaves on newly emerging tillers of the wsl4 mutant also exhibited the green/white leaf color defect. No difference was observed between WT and wsl4 mutant after the heading. Consistent with these observations, levels of Chl a and b in striped leaves of wsl4 plants were much lower than those in WT. Compared with WT plants, wsl4 mutants showed no statistically significant differences in plant height, number of tillers, number of branches per panicle, number of spikelets per panicle, and 1000-grain weight. In contrast to the moderate striped phenotype and Chl accumulations under optimum temperature (L30/D25), the wsl4 mutant exhibited less color deficiency and accumulated much higher Chl contents when grown at a constant 30°C (C30). At a constant 20°C the wsl4 mutant showed more extreme symptoms and Chl content that were barely detectable.LeafThe third leaf at the three-leaf stageRT-PCR products were sequenced90.00%HighIncreased28694820
RX69ComComplementationFor complementation of the wsl4 mutation a 5.7 kb WT genomic fragment containing a 2.2 kb upstream sequence, the entire coding region of WSL4, and a 2.0 kb downstream sequence was amplified from RX69 Recessive; HomozygousGreen leafLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced20.00%LowRestored28694820
RX69RNAiRNA interferenceFor RNAi test, the construct pCUbi1390-DFAD2 (ubiquitin promoter and a FAD2 intron inserted into pCAMBIA1390) was used as an RNAi vector (Stoutjesdijk et al., 2002; Wu et al., 2007). Both anti-sense aNAWhite leafLeafThe third leaf at the three-leaf stageRT-PCR products were sequenced99.00%HighIncreased28694820
Last update: Jul 2021 (version 1.0)