WSP1 - Plant Editosome Database

Summary

Editing Factor:	WSP1
Synonym:	NA
Description:	Transmission electron microscopy analysis and chlorophyll content measurement revealed a chloroplast developmental defect and reduced chlorophyll accumulation in wsp1; WSP1 is localized to chloroplasts and the point mutation in wsp1 affected the editing of multiple organellar RNA sites; Owing to the defect in plastid RNA editing, chloroplast ribosome biogenesis and ndhA splicing were also impaired in wsp1, which may affect normal chloroplast development in the leaves and panicles at the heading stage
Protein Family:	Multiple organellar RNA editing factor (MORF)
Subclass:	NA
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	LOC_OS04G51280 (Oryza sativa)
Edited Gene(s):	rpoB rps14 ndhG ndhD
Editing Type(s):	C-to-U (12)
Publication(s):	[1] The RNA Editing Factor WSP1 Is Essential for Chloroplast Development in Rice, Molecular Plant, 2017. [doi:10.1016/j.molp.2016.08.009]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_OS04G51280

Chloroplast

ndhD

878

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591

Oryza sativa

LOC_OS04G51280

Chloroplast

ndhG

-11

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591

Oryza sativa

LOC_OS04G51280

Chloroplast

rpoB

467

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591

Oryza sativa

LOC_OS04G51280

Chloroplast

rpoB

545

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591

Oryza sativa

LOC_OS04G51280

Chloroplast

rpoB

560

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591

Oryza sativa

LOC_OS04G51280

Chloroplast

rps14

CDS

Rice Genome Annotation Project (http://rice.uga.edu/)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	wsp1	T-DNA Insertion	The T-DNA insertion causes a single base change in the third one within exon 3 (Leu - His )	Homozygous	White-stripe leaves and panicles, a chloroplast developmental defect and reduced chlorophyll accumulation	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27622591
Japonica cultivar Nipponbare	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	NA	High	None	27622591