YS1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: YS1
Synonym: YELLOW SEEDLING 1
Description: YS1 is absolutely required for editing of site 25992 in rpoB transcripts encoding the beta subunit of the plastid-encoded RNA polymerase (PEP); The activity of PEP containing RpoB translated from unedited transcripts is insufficient to support rapid chloroplast differentiation
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-DYW
Gene ID & Species: AT3G22690 (Arabidopsis thaliana)
Edited Gene(s): rpoB
Editing Type(s): C-to-U (9)
Publication(s): [1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114]
[2] The Arabidopsis gene YS1 encoding a DYW protein is required for editing of rpoB transcripts and the rapid development of chloroplasts during early growth, The Plant Journal, 2009. [doi:10.1111/j.1365-313X.2008.03766.x]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G22690 Chloroplast rpoB 338 CDS NA NA NA NA C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlNo mutantNormalSeedling6 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTControlControlNo mutantNormalSeedling30 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTControlControlNo mutantNormalSeedling5 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0ys1T-DNA InsertionNAHomozygousYellow seedlingSeedling6 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling30 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling5 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0WTControlControlNo mutantNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract74.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighIncreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighIncreased28761003
Last update: Sep 2026 (version 2.0)