YS1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: YS1
Synonym: YELLOW SEEDLING1
Description: Encoding a PPR protein involved in RNA editing of plastid encoded genes; Natural variation in this locus is associated with increased photosynthetic acclimation.
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-DYW
Gene ID & Species: AT3G22690 (Arabidopsis thaliana)
Edited Gene(s): rpoB
Editing Type(s): C-to-U (10)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The Arabidopsis gene YS1 encoding a DYW protein is required for editing of rpoB transcripts and the rapid development of chloroplasts during early growth., The Plant journal : for cell and molecular biology, 2009. [PMID=19054358]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G22690 Chloroplast
Plastid
rpoB 338 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalSeedling6 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTControlControlControlNormalSeedling30 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTControlControlControlNormalSeedling5 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0ys1T-DNA InsertionNANull allelesYellow seedlingSeedling6 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling30 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling5 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0WTControlControlControlNANANARNA-seq76.41%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract74.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighIncreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighIncreased28761003
Last update: Feb 2026 (version 2.0)