YS1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: YS1
Synonym: YELLOW SEEDLING1
Description: Encodes a PPR protein involved in RNA editing of plastid encoded genes. Natural variation in this locus is associated with increased photosynthetic acclimation.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: AT3G22690 (Arabidopsis thaliana)
Edited Gene(s): rpoB
Editing Type(s): C-to-U (10)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The Arabidopsis gene YS1 encoding a DYW protein is required for editing of rpoB transcripts and the rapid development of chloroplasts during early growth, The Plant Journal, 2009. [PMID=19054358]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G22690 Chloroplast
Plastid
rpoB 338 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling6 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling30 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling5 daysDirect Sequencing of PCR Products100.00%CompleteNone19054358
Col-0ys1T-DNA InsertionNANull allelesYellow seedlingSeedling6 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling30 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0ys1T-DNA InsertionNANAYellow seedlingSeedling5 daysDirect Sequencing of PCR Products0.00%UneditedAbsent19054358
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq76.41%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract74.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighIncreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighIncreased28761003
Last update: Jul 2021 (version 1.0)