Accession

PRJCA009996

Title

A Fuzzy sequencer for rapid DNA fragment counting and genotyping

Relevance

Methodology

Data types

Whole genome sequencing

Organisms

Severe acute respiratory syndrome coronavirus 2
human oral metagenome
Mus musculus
Escherichia phage Lambda
human lung metagenome
Homo sapiens
human feces metagenome

Description

High-throughput sequencing technologies generate a vast number of DNA sequence reads simultaneously, which are subsequently analyzed using the information contained within these fragmented reads. The assessment of sequencing technology relies on information efficiency, which measures the amount of information entropy produced per sequencing reaction cycle. In this study, we propose a fuzzy sequencing strategy that exhibits information efficiency more than twice of currently prevailing cyclic reversible terminator sequencing methods. To validate our approach, we developed a fully functional and high-throughput fuzzy sequencer. This sequencer implements a highly efficient fluorogenic sequencing-by-synthesis chemistry and underwent testing across various application scenarios, including copy-number variation detection, noninvasive prenatal testing, transcriptome profiling, mutation genotyping, and metagenomic profling. Our findings unequivocally demonstrate that the fuzzy sequencing strategy outperforms existing methods in terms of information efficiency and delivers accurate resequencing results with faster turnaround times.

Sample scope

Multiisolate

Release date

2025-03-29

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)		21927802
National Natural Science Foundation of China (NSFC)		22050002
National Natural Science Foundation of China (NSFC)		T2188102
Beijing Municipal Science and Technology Commission		Z201100005320016
Beijing Advanced Innovation Center for Genomics		-
National Natural Science Foundation of China (NSFC)		T2225005

Submitter

Yanyi Huang (yanyi@pku.edu.cn)

Organization

Peking University

Submission date

2022-06-10

Project Data

Resource name	Description
BioSample (76) show	-
SAMC4920528	Patient 54
SAMC4920527	Patient 53
SAMC4920526	Patient 52
SAMC4920525	Patient 51
SAMC4920524	Patient 50
SAMC4920523	Patient 49
SAMC4920522	Patient 48
SAMC4920521	Patient 47
SAMC4920520	Patient 46
SAMC4920519	Patient 45
SAMC4920518	Patient 44
SAMC4920517	Patient 43
SAMC4920516	Patient 42
SAMC4920515	Patient 41
SAMC4920514	Patient 40
SAMC4920513	Patient 39
SAMC4920512	Patient 38
SAMC4920511	Patient 37
SAMC4920510	Patient 36
SAMC4920509	Patient 35
SAMC4920508	Patient 34
SAMC4920507	Patient 33
SAMC4920506	Patient 32
SAMC4920505	Patient 31
SAMC4920504	Patient 30
SAMC4920503	Patient 29
SAMC4920502	Patient 28
SAMC4920501	Patient 27
SAMC4920500	Patient 26
SAMC4920499	Patient 25
SAMC4920498	Patient 24
SAMC4920497	Patient 23
SAMC4920496	Patient 22
SAMC4920495	Patient 21
SAMC4920494	Patient 20
SAMC4920493	Patient 19
SAMC4920492	Patient 18
SAMC4920491	Patient 17
SAMC4920490	Patient 16
SAMC4920489	Patient 15
SAMC4920488	Patient 14
SAMC4920487	Patient 13
SAMC4920486	Patient 12
SAMC4920485	Patient 11
SAMC4920484	Patient 10
SAMC4920483	Patient 9
SAMC4920482	Patient 8
SAMC4920481	Patient 7
SAMC4920480	Patient 6
SAMC4920479	Patient 5
SAMC4920478	Patient 4
SAMC4920477	Patient 3
SAMC4920476	Patient 2
SAMC4920475	Patient 1
SAMC4920474	SARS-CoV-2 isolates from E135
SAMC4920473	SARS-CoV-2 isolates from E80
SAMC4920472	SARS-CoV-2 isolates from E20
SAMC4920471	lambda phage
SAMC4920470	CAP 14
SAMC4920469	CAP 13
SAMC4920468	CAP 12
SAMC4920467	CAP 11
SAMC4920466	CAP 10
SAMC4920465	CAP 9
SAMC4920464	CAP 8
SAMC4920463	CAP 7
SAMC4920462	CAP 6
SAMC4920461	CAP 5
SAMC4920460	CAP 4
SAMC4920459	CAP 3
SAMC4920458	CAP 2
SAMC4920457	CAP 1
SAMC4920456	Pediatric patient, throat swab
SAMC4920455	Pediatric patient, anal swab
SAMC4920454	MEF
SAMC4920453	mES
GSA (4)	-
CRA024248	Covid-19 of Fuzzy-seq
CRA024247	Lambda phage sequencing of Fuzzy-seq
CRA024246	Metagenomic sequencing of Fuzzy-seq
CRA024244	RNA-seq of mouse cell lines
GSA-Human (1)	-
HRA010915	A Fuzzy sequencer for fast DNA fragment counting and genotyping

Project Data

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