项目编号

PRJCA012020

项目标题

SARS-CoV-2 breakthrough infections induce somatically hypermutated broadly neutralizing antibodies against heterologous variants

涉及领域

Disease Study

数据类型

Transcriptome or Gene expression
Single cell sequencing

物种名称

Homo sapiens

描述信息

Currently circulating SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading acute-infection-induced germline antibodies isolated earlier in the pandemic. We identified that memory B cells from Delta variant breakthrough-infection patients expressed antibodies with more extensive somatic hypermutations (SHMs) allowing isolation of a number of broadly neutralizing antibodies with activities against heterologous variants of concerns (VOCs) including Omicron variant. Identified SHMs involved heavily in epitope recognition, they broadened neutralization breadth by rendering antibodies resistant to VOC mutations highly detrimental to previously isolated antibodies targeting similar epitopes. These data provide molecular mechanisms for enhanced immunity to heterologous SARS-CoV-2 variants after repeated antigen exposures with implications for future vaccination strategy.

样品范围

Single cell

发布日期

2023-01-15

项目资金来源

机构	项目类型	授权项目ID	授权项目名称
Emergency Key Program of Guangzhou Laboratory		EKPG21-29
Emergency Key Program of Guangzhou Laboratory		EKPG21-31

提交者

Feng Li (gz8h_lifeng@126.com)

提交单位

Guangzhou Eighth People's Hospital, Guangzhou Medical University

提交日期

2022-09-20

项目包含数据信息

资源名称	描述
BioSample (37) show	-
SAMC2331952	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331946	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331944	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331939	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331935	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331928	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331925	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331921	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331917	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331913	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331909	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331905	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331901	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331899	scBCR-Seq of human PBMC: Delta-breakthrough patients
SAMC2331895	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331893	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331888	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331885	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331876	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331872	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331868	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331865	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331861	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331859	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331855	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331851	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331849	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331844	scRNA-Seq of human PBMC: Delta-breakthrough patients
SAMC2331841	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331837	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331834	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331831	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331827	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331822	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331818	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331814	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine
SAMC2331810	scBCR-Seq of human PBMC: COVID-19 infected-only patients without vaccine