e.g., PRJCA000229; PRJNA784038; human
Accession PRJCA012020
Title SARS-CoV-2 breakthrough infections induce somatically hypermutated broadly neutralizing antibodies against heterologous variants
Relevance Disease Study
Data types Transcriptome or Gene expression
Single cell sequencing
Organisms Homo sapiens
Description Currently circulating SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading acute-infection-induced germline antibodies isolated earlier in the pandemic. We identified that memory B cells from Delta variant breakthrough-infection patients expressed antibodies with more extensive somatic hypermutations (SHMs) allowing isolation of a number of broadly neutralizing antibodies with activities against heterologous variants of concerns (VOCs) including Omicron variant. Identified SHMs involved heavily in epitope recognition, they broadened neutralization breadth by rendering antibodies resistant to VOC mutations highly detrimental to previously isolated antibodies targeting similar epitopes. These data provide molecular mechanisms for enhanced immunity to heterologous SARS-CoV-2 variants after repeated antigen exposures with implications for future vaccination strategy.
Sample scope Single cell
Release date 2023-01-15
Grants
Agency program Grant ID Grant title
Emergency Key Program of Guangzhou Laboratory EKPG21-29
Emergency Key Program of Guangzhou Laboratory EKPG21-31
Submitter Feng Li (gz8h_lifeng@126.com)
Organization Guangzhou Eighth People's Hospital, Guangzhou Medical University
Submission date 2022-09-20

Project Data

Resource name Description
BioSample (37)  show -
GSA-Human (1)-
HRA003371Single B cell sequencing data for patients with SARS-CoV-2 infections