Accession PRJCA016185
Title Early detection and prognosis evaluation for hepatocellular carcinoma by circulating tumor DNA methylation: A multicenter cohort study
Relevance Medical
Data types Epigenomics
Organisms Homo sapiens
Description Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide, and early detection is crucial for improving patient survival. We aimed to develop a blood-based assay to aid HCC early detection. We conducted a three-phase, multi-center study to screen, optimize and validate HCC-specific differentially methylated regions (DMRs) using quantitative methylation-specific PCR (qMSP). Genomic-wide profiling of DNA methylation identified DMRs in HCC tumors distinguished from normal tissues and healthy plasmas. Twenty most effective DMRs were verified and incorporated into a multi-locus qMSP assay (HepaAiQ). The HepaAiQ model was trained for HCC early detection with a multi-center cohort of 559 plasmas of 293 HCC patients (76.5% stage 0/A) and 266 controls including 96 chronic hepatitis B (CHB) or liver cirrhosis (LC), 23 benign hepatic lesion (BHL) and 147 healthy controls (HC). Blind validation of HepaAiQ in an independent cohort of 523 participants showed consistent performance with a sensitivity of 84.4% in 205 HCC (81.5% stage 0/A) and a specificity of 90.3% in 318 controls (100 CHB/LC, 102 BHL and 116 HC). HepaAiQ significantly outperformed commonly used serum markers in the same cohort. Moreover, HepaAiQ was evaluated in paired pre/postoperative plasmas from 103 HCC patients and correlated with 2-year patient outcomes. Patients with postoperative positive HepaAiQ exhibit higher recurrent risk than negatives, suggesting a prognostic value of HepaAiQ.HepaAiQ, a non-invasive qMSP assay, is developed to accurately measure HCC-specific DMRs and shows great potentials for HCC early detection and prognosis to benefit the population at risk.
Sample scope Multiisolate
Release date 2023-04-11
Publication
PubMed ID Article title Journal name DOI Year
38741204 Early detection and prognosis evaluation for hepatocellular carcinoma by circulating tumour DNA methylation: A multicentre cohort study Clinical and translational medicine 10.1002/ctm2.1652 2024
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2019YFC1315800
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2019YFC1315801
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2021YFC2501900
National Natural Science Foundation of China (NSFC) the Original Discovery Program of National Natural Science of China 82150004
National Natural Science Foundation of China (NSFC) 82072715
National Natural Science Foundation of China (NSFC) 82341027
Shanghai Municipal Health Commission the Shanghai Municipal Health Commission Collaborative Innovation Cluster Project 2019CXJQ02
Shanghai Municipal Health Commission 2022LJ005
Shanghai Science and Technology Commission 21140900300
Submitter jian zhou (dezhen96@163.com)
Organization Zhongshan Hospital, Fudan University
Submission date 2023-04-11

Project Data

Resource name Description