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项目编号 PRJCA030053
项目标题 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions
涉及领域 Agricultural
数据类型 Epigenomics
Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
物种名称 Bos taurus
描述信息 While DNA methylation in other tissues can be approximated through model species, the dynamic distribution and regulatory significance of DNA methylation in the rumen, a unique organ in ruminant, remain largely unknown. Here, we employed whole-genome bisulfite sequencing (WGBS), transcriptomics, and histone modification data to compare fetal and adult stages of bovine rumen with other tissues, including pluripotent stem cells (PSCs) approximating pre-implantation embryos. We found extensive methylation differences, including CG methylation (mCG) and non-CG methylation (mCH; H represents A, C and T) between the rumen at fetal and adult stages and other tissues and PSCs. These differentially methylated regions (DMRs) are closely associated with other epigenetic regulatory components, such as transcription factors (TFs) and histone modifications. These DMRs can also combine to form large hypo CG-DMRs to regulate a cluster of functionally related genes. We elucidated the reasons for morphological and functional differences between fetal and adult rumen at the epigenetic level and the interactions between epigenetic modifications and gene expression. This study highlights the differences in methylation patterns between the rumen and other tissues during development and the role of DNA methylation in controlling gene expression and establishing tissue-specific functions.
样品范围 Multiisolate
发布日期 2024-09-10
出版信息
PubMed ID 文章标题 杂志名称 Doi 发表年份
39572019 Whole-genome methylation reveals tissue-specific differences in non-CG methylation in bovine Zoological Research 10.24272/j.issn.2095-8137.2024.221 2024
项目资金来源
机构 项目类型 授权项目ID 授权项目名称
Education Department of Inner Mongolia Autonomous Region General Program NMGIRT2204 Research on the Maintenance Mechanism and Differentiation Ability of Pluripotent Stem Cells in Grassland Livestock
National Natural Science Foundation of China (NSFC) International (Regional) Cooperation and Exchange Program 32160172 Establishing an efficient differentiation system for inducing SSCs formation in bovine EPSCs using PLZF reporting system
本项目在其他数据库系统中的项目序列号
项目序列号 数据库名称
GSE264346 NCBI
提交者 jing wang (nnlrl@mail.imu.edu.cn)
提交单位 Inner Mongolia University
提交日期 2024-09-10

项目包含数据信息

资源名称 描述
BioSample (48)  show -
GSA (5) -
CRA019132 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions (RNA-seq)
CRA019386 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions
CRA018995 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions (BS-seq)
CRA018940 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions (cut&tag-seq)
CRA018938 Epigenetic Basis for the Establishment of Ruminal Tissue-Specific Functions (ATAC-seq)