Protocols: In vitro culture protocol for human gallbladder cells. Dissociated cells from human gallbladder and cystic duct were cultured in rat mammary feeder culture using a modified culture medium as published in PMID 23562832. Adenoviral transduction of expanded/cultured human gallbladder cells and Reprogramming culture conditions of transduced human gallbladder cells Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
Sample type
Generic.1.0
Attributes
phenotype
Hpi1 positive
ENA-CHECKLIST
ERC000011
INSDC first public
2017-08-02T17:01:48Z
sample name
E-MTAB-5556:Hpi1pos_b2t1
scientific_name
Homo sapiens
cell type
epithelial cell of gall bladder reprogrammed to beta cell
