Data Statistics and tSNE/UMAP Visualization

  • Overview of the dataset
  • tSNE/UMAP - unsupervised clustering
  • The dataset included a total of 735 cells, the parameters adopted were as follows: resolution=1, PCs=1:20, MT<10%.

Tumor Microenvironment

  • Cell components
  • Subtype Cell Count Percentage
    CD4+ naive T cells 2 0.270%
    CD4+ Tcm 11 1.500%
    CD4+ Tem 1 0.140%
    Tregs 2 0.270%
    CD8+ naive T cells 4 0.540%
    CD8+ effector T cells 2 0.270%
    CD8+ Tcm 4 0.540%
    CD8+ Tem 13 1.770%
    NK cells 10 1.360%
    Naive B cells 12 1.630%
    Activated B cells 2 0.270%
    Memory B cells 2 0.270%
    Plasma cells 1 0.140%
    Macrophages 25 3.400%
    Monocytes 179 24.350%
    Dendritic cells 10 1.360%
    GMPs 75 10.200%
    Unassigned 380 51.700%
  • Differential expression analysis
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    Gene Symbol
    Avg_logFC
    Pct.1
    Pct.2
    p-value
    adj_pvalue
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  • GO and KEGG enrichments

Functional Genes' Expression

  • Receptor and ligand genes' expression
  • Receptor genes and their paired ligand genes were collected from CelltalkDB, SingleCellSingalR, Cellinker, Cell-Cell Interaction Database and Armingol E, et al., 2021. The following figures only demonstrated the expression of receptor genes and ligand genes supported by three or more databases or other sources, and the corresponding relationship between ligands and receptors were shown in the following table.

  • Oncogenes and suppressor genes' expression
  • Oncogenes and tumor suppressor genes (TSGs) were collected from Cancer Gene Census (CGC), OncoKB, Network of Cancer Genes (NCG), TSGene, IntOGene and Volkan Okur and Wendy K. Chung, 2017. The following figures only displayed the expression of oncogenes and TSGs supported by three or more databases or other sources.