生长方案:	For every embryo, the main organs of digest system, including esophagus, stomach, small intestine (SI), large intestine (LI), were dissected. For 11W to 25W small intestine, the villi and crypt were separated by shaving off the villi carefully with a surgical blade. The mesenterium of small intestine and large intestine were torn off carefully under a microscope. The middle section of dissected esophagus, SI and LI was used to collect single cells. And the corpus of stomach was used to collect single cells. The selected tissue pieces were washed by PBS for several times considering there many microorganisms and mucus in digest system even in the early development stage. Then the selected tissue pieces were minced to small pieces by surgical scissors, after which the minced tissues were digested to single cells by using 2mg/mL Collagenase/Dispase for 30-40 min at 37. When digest intestine crypt, 2mM EDTA was firstly used to insure the release of the cells from intestine crypt. 40m strainer was used to filter large tissue debris. Then the cell suspension in the solution of Collagenase/Dispase was centrifuged at 800g for 5min at 4 and the cell pellet was re-suspended in 1% HSA.
处理方案:	-
提取方案:	After pick single cells using mouth pipette, they were transferred to cold scRNA-seq lysis buffer immediately. Subsequently, the lysis buffer were shocked for 3min at 72 to make sure the release of RNA.
建库方案:	-

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	Reverse; -
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 4000
链特异性:	Specific; Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度