概要: The rice gene SUB1A-1 confers flooding tolerance restricting shoot growth during submergence. Rice with SUB1A also show more rapid recovery after submergence ends, but mechanisms by which SUB1A improves recovery from submergence had not been examined. In this study, the transcriptome was sequenced at five time points over a 24 hour submergence recovery period in near-isogenic rice genotypes with and without SUB1A.
项目整体设计: 36 samples, 2 genotypes: M202 and 202(Sub1), 2 conditions: unsubmerged and 3d whole shoot submergence, 5 time points: desubmergence (at midday following 3d submergence), dusk, midnight, dawn, midday (24 hours after desubmergence). 3 bioreplicates. Unsubmerged plants only sequenced for at desubmergence time point.
Rice seeds (Oryza sativa L. ssp. japonica) of cultivar M202 and its near-isogenic, Sub1 introgression line, M202(Sub1), were sterilized for 30 min in a solution of 5% (v/v) bleach and 0.01% (v/v) Tween-20, rinsed and soaked overnight in DI water. After germinating in covered glass dishes on wet paper towels for 5 d, seedlings were transplanted into 10 cm pots in the greenhouse at a planting density of 20 plants per pot. Plants were grown in the greenhouse for 12 d before beginning the submergence treatment.
处理方案:
Plants were submerged in 121 L bins, in which water was allowed to equilibrate to ambient temperature for 24 h before submergence. Submergence began at ZT 6:00 and continued for 72 h, at which time plants were placed back on the greenhouse bench for reoxygenation. Whole shoots were harvested and flash-frozen in liquid N2 immediately upon desubmergence (3d_sub) at midday (ZT9:00), at dusk (ZT15:30), midnight (ZT19:45), dawn (ZT0), and again at midday 24 h following desubmergence (ZT9:00).
提取方案:
Total RNA was isolated from frozen shoot tissue with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany).
建库方案:
cDNA libraries for each sample were prepared from purified mRNA according to Wang et al. (2011).
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500
链特异性:
Specific
样本
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样本描述:
生物条件:
实验变量:
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质量评估:
数据来源
GEN样本编号
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物种
种族
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来源名称
组织
细胞类型
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细胞系
疾病
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发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标(Spike-In)
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平均测序片段长度_1
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唯一比对率
多重比对率
覆盖度
文章
Rice SUB1A constrains remodelling of the transcriptome and metabolome during submergence to facilitate post-submergence recovery.