Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA397883: mRNA sequencing of whole 15 d old Oryza sativa shoots

来源: NCBI / GSE102494
提交时间: Aug 10 2017
释放时间: Sep 01 2017
最后更新时间: May 15 2019

概要: The rice gene SUB1A-1 confers flooding tolerance restricting shoot growth during submergence. Rice with SUB1A also show more rapid recovery after submergence ends, but mechanisms by which SUB1A improves recovery from submergence had not been examined. In this study, the transcriptome was sequenced at five time points over a 24 hour submergence recovery period in near-isogenic rice genotypes with and without SUB1A.

项目整体设计: 36 samples, 2 genotypes: M202 and 202(Sub1), 2 conditions: unsubmerged and 3d whole shoot submergence, 5 time points: desubmergence (at midday following 3d submergence), dusk, midnight, dawn, midday (24 hours after desubmergence). 3 bioreplicates. Unsubmerged plants only sequenced for at desubmergence time point.

GEN 数据集:
GEND000261
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方案
生长方案: Rice seeds (Oryza sativa L. ssp. japonica) of cultivar M202 and its near-isogenic, Sub1 introgression line, M202(Sub1), were sterilized for 30 min in a solution of 5% (v/v) bleach and 0.01% (v/v) Tween-20, rinsed and soaked overnight in DI water. After germinating in covered glass dishes on wet paper towels for 5 d, seedlings were transplanted into 10 cm pots in the greenhouse at a planting density of 20 plants per pot. Plants were grown in the greenhouse for 12 d before beginning the submergence treatment.
处理方案: Plants were submerged in 121 L bins, in which water was allowed to equilibrate to ambient temperature for 24 h before submergence. Submergence began at ZT 6:00 and continued for 72 h, at which time plants were placed back on the greenhouse bench for reoxygenation. Whole shoots were harvested and flash-frozen in liquid N2 immediately upon desubmergence (3d_sub) at midday (ZT9:00), at dusk (ZT15:30), midnight (ZT19:45), dawn (ZT0), and again at midday 24 h following desubmergence (ZT9:00).
提取方案: Total RNA was isolated from frozen shoot tissue with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany).
建库方案: cDNA libraries for each sample were prepared from purified mRNA according to Wang et al. (2011).
测序信息
分子类型: poly(A)+ RNA
库的片段类型: PAIRED
库的链类型: Forward
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 2500
链特异性: Specific
样本
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数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
Rice SUB1A constrains remodelling of the transcriptome and metabolome during submergence to facilitate post-submergence recovery.
Plant, cell & environment . 2017-11-27 [PMID: 29094353]